Identification of marker genes for pars tuberalis morphogenesis in chick embryo: expression of Cytokine-like 1 and Gap junction protein alpha 5 in pars tuberalis.
Identification of marker genes for pars tuberalis morphogenesis in chick embryo: expression of Cytokine-like 1 and Gap junction protein alpha 5 in pars tuberalis.
复制标题
鸡胚结节形态发生标记基因的鉴定：细胞因子样1和间隙连接蛋白α5在结节中的表达。
DOI：
10.1007/s00441-016-2484-9
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Sakata I and Sakai T
中科院分区：
文献类型：
--
作者：
Aizawa S;Higaki Y;Dudaui A;Nagasaka M;Takahashi S;Sakata I and Sakai T
腺垂体由口腔外胚层形成，由远侧部（PD）、中间部和结节部（PT）组成。PD发展的机制已被广泛研究，并且PD的细胞分化也被很好地理解。然而，PT的形态发生和分化仍然不清楚，PT发育过程中表达的基因在很大程度上仍然未知。我们探索了胚胎发育过程中PT特异性表达的基因，并分析了它们的时空表达模式。从胚胎第10天（E10.0）的鸡胚中获得的激光捕获PT和PD组织的微阵列分析显示PT中细胞因子样1（CYTL1）和间隙连接蛋白α 5（GJA5）基因的高表达。通过原位杂交对这些时空表达模式的详细分析表明，CYTL1mRNA最早出现在E1.5的侧头外胚层和腹头外胚层。在E2.5时，CYTL1的表达进入Rathke囊，然后定位于PT原基，在那里它持续表达直到E12.0。GJA5 mRNA在PT原基中从E6.0到E12.0瞬时检测到，而在发育过程中的PD中未检测到其表达。因此，这些基因可能参与PT发生的调控机制，并可能成为PT发生的有用标记。
The adenohypophysis is formed from the oral ectoderm and consists of the pars distalis (PD), pars intermedia, and pars tuberalis (PT). The mechanisms of PD development have been extensively studied, and the cellular differentiation of the PD is well understood. However, the morphogenesis and differentiation of the PT are still unclear, and the genes expressed during PT development remain largely unknown. We have explored genes specifically expressed in the PT during embryonic development and analyzed their spatiotemporal expression patterns. Microarray analysis of laser-captured PT and PD tissues obtained from chick embryos on embryonic day 10 (E10.0) has shown high expression of Cytokine-like 1 (CYTL1) and Gap junction protein alpha 5 (GJA5) genes in the PT. Detailed analysis of these spatiotemporal expression patterns during chick embryo development by in situ hybridization has revealed thatCYTL1mRNA first appears in the lateral head ectoderm and ventral head ectoderm at E1.5. The expression ofCYTL1moves into Rathke’s pouch at E2.5 and is then localized in the PT primordium where it is continuously expressed until E12.0.GJA5mRNA is transiently detected in the PT primordium from E6.0 to E12.0, whereas its expression is not detected in the PD during development. Thus, these genes might be involved in the regulation mechanisms of PT development and could be useful markers for PT development.