Special structure of mitochondrial DNA control region and phylogenetic relationship among individuals of the black rockfish, Sebastes schlegelii
Special structure of mitochondrial DNA control region and phylogenetic relationship among individuals of the black rockfish, Sebastes schlegelii
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石斑鱼线粒体DNA控制区的特殊结构及个体间的系统发育关系
DOI：
10.3109/19401736.2012.731401
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发表时间：
2013-04-01
影响因子：
--
通讯作者：
Gao, Tianxiang
中科院分区：
文献类型：
--
作者：
Zhang, Hui;Zhang, Yan;Gao, Tianxiang
对许氏平鲉线粒体DNA控制区（CR）的结构进行了研究。结果表明，该菌株存在两个末端相关序列（塔斯）、两个互补末端相关序列（cTAS）和保守序列区（CSB），如CSB F、CSB E、CSB D、CSB 1、CSB 2和CSB 3。施莱格利。结果表明，这些块体的结构与大多数海洋鱼类相似，但其特殊之处在于S的CR中有两个塔斯和两个cTAS。施莱格利。从450 bp到CR末端有一个保守区，这也是S.施莱格利。CSB 1、CSB 2和CSB 3区的所有序列在不同个体之间是一致的，这与大多数脊椎动物有很大不同。此外，还利用线粒体DNA的CR全序列和CR前449 bp的序列分析了S.施莱格利。系统发育树显示个体间缺乏遗传结构。本研究还表明，野生个体和养殖个体的遗传多样性可能相似，这可能有助于渔业管理。
This study deals with the structure of mitochondrial DNA (mtDNA) control region (CR) of the black rockfish, Sebastes schlegelii. Two termination-associated sequences (TASs), two complementary termination-associated sequences (cTASs), and conserved sequence block (CSB), such as CSB-F, CSB-E, CSB-D, CSB1, CSB2, and CSB3, were detected in S. schlegelii. The results indicated that the structures of these blocks are similar to most marine fishes, but it is special that there are two TASs and two cTASs in the CR of S. schlegelii. One conserved region was found from 450 bp to the end of the CR, which is also a special feature of S. schlegelii. All sequences of CSB1, CSB2, and CSB3 blocks are the consensus among different individuals, which is quite different from most vertebrates. In addition, the complete mtDNA CR sequences and the first 449 bp of the CR are used to analyze the phylogenetic relationships of S. schlegelii. The phylogenetic trees show a lack of genetic structure among individuals. This study also indicated a signal that the genetic diversity might be similar between the wild and cultured individuals, which may be helpful to the fisheries management.