Response of MAPK pathway to iron oxide nanoparticles in vitro treatment promotes osteogenic differentiation of hBMSCs
Response of MAPK pathway to iron oxide nanoparticles in vitro treatment promotes osteogenic differentiation of hBMSCs
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DOI：
10.1016/j.biomaterials.2016.02.004
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发表时间：
2016-04-01
期刊：
影响因子：
14
通讯作者：
Gu, Ning
中科院分区：
文献类型：
--
作者：
Wang, Qiwei;Chen, Bo;Gu, Ning
氧化铁纳米颗粒（IONP）通常用于多种生物医学应用。本课题组前期研究已证实了IONP的组织修复作用，但其机制尚不清楚。干细胞疗法在组织工程和再生医学领域具有广阔的前景，但IONP是否能调节干细胞命运，促进组织修复尚不清楚。在此，我们发现IONP可以促进人骨源性间充质干细胞（hBMSCs）在体外的成骨分化。为了深入了解其分子机制，我们利用基因芯片和生物信息学分析方法进行了系统分析，结果显示IONP处理后基因表达受到广泛调控，经典的丝裂原活化蛋白激酶（MAPK）信号通路被激活。因此，调控该通路的下游基因以促进成骨分化。总之，本研究阐明了解释IONP如何影响hBMSCs的分子基础，这可能对干细胞在再生医学中的应用产生许多有意义的影响。(C)2016爱思唯尔有限公司版权所有
Iron oxide nanoparticles (IONPs) are generally used in multiple biomedical applications. The tissue repair effect of IONPs had been demonstrated in the previous studies of our group, but the underlying mechanism is unclarified. It is well known that stem cell-based therapies show promising prospect in tissue engineering and regenerative medicine, however, whether IONPs could modulate stem cell fate to promote tissue repair is still unclear. Herein, we found that IONPs could promote osteogenic differentiation of human bone-derived mesenchymal stem cells (hBMSCs) in vitro. To insightfully understand the molecular mechanisms, we performed systematic analyses by use of gene microarray assay and bioinformatics analysis, which revealed that gene expression was widely regulated and classical mitogen-activated protein kinase (MAPK) signal pathway was activated by IONPs treatment. As a result, downstream genes of this pathway were regulated to promote osteogenic differentiation. In summary, the present study elucidates a molecular basis explaining how IONPs effect on hBMSCs, which could have many meaningful impacts for stem cells application in regenerative medicine. (C) 2016 Elsevier Ltd. All rights reserved.