Characterization of a Novel Subtilisin-like Protease Myroicolsin from Deep Sea Bacterium Myroides profundi D25 and Molecular Insight into Its Collagenolytic Mechanism
Characterization of a Novel Subtilisin-like Protease Myroicolsin from Deep Sea Bacterium Myroides profundi D25 and Molecular Insight into Its Collagenolytic Mechanism
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来自深海细菌 Myroides profundi D25 的新型枯草杆菌蛋白酶样蛋白酶 myroicolsin 的表征及其胶原蛋白溶解机制的分子洞察
DOI：
10.1074/jbc.m113.513861
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发表时间：
2014-02-28
影响因子：
4.8
通讯作者：
Zhang, Xi-Ying
中科院分区：
文献类型：
--
作者：
Ran, Li-Yuan;Su, Hai-Nan;Zhang, Xi-Ying
背景：海洋胶原蛋白降解的机制在很大程度上是未知的。结果：鉴定了一种来源于海洋细菌的类枯草杆菌蛋白酶Myroicolsin，并对其胶原溶解机制进行了初步研究。结论：Myroicolsin与其他类枯草杆菌蛋白酶相比，具有新颖的结构域和独特的胶原降解机制。重要性：该研究为枯草杆菌蛋白酶样蛋白酶溶解胶原蛋白和海洋氮循环的机制提供了新的见解。胶原蛋白是一种不溶性蛋白质，广泛分布于海洋动物的细胞外基质中。胶原蛋白降解是海洋氮循环的重要步骤。然而，海洋胶原蛋白降解的机制在很大程度上仍然是未知的。本文对深海细菌Myroides profundi D25分泌的一种新的类枯草杆菌蛋白酶myroicolsin进行了纯化和鉴定，并对其胶原溶解机制进行了研究。Myroicolsin显示低身份（
Background: The mechanism of marine collagen degradation is largely unknown. Results: Myroicolsin, a subtilisin-like protease from a marine bacterium, was characterized, and its collagenolytic mechanism was studied. Conclusion: Myroicolsin has a novel domain structure and a unique collagen degradation mechanism compared with other subtilisin-like proteases. Significance: This study provides new insights into the mechanism of subtilisin-like proteases' collagenolysis and marine nitrogen cycling.Collagen is an insoluble protein that widely distributes in the extracellular matrix of marine animals. Collagen degradation is an important step in the marine nitrogen cycle. However, the mechanism of marine collagen degradation is still largely unknown. Here, a novel subtilisin-like collagenolytic protease, myroicolsin, which is secreted by the deep sea bacterium Myroides profundi D25, was purified and characterized, and its collagenolytic mechanism was studied. Myroicolsin displays low identity (