High MERS-CoV seropositivity associated with camel herd profile, husbandry practices and household socio-demographic characteristics in Northern Kenya.
High MERS-CoV seropositivity associated with camel herd profile, husbandry practices and household socio-demographic characteristics in Northern Kenya.
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DOI：
10.1017/s0950268820002939
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发表时间：
2020-12-01
影响因子：
4.2
通讯作者：
Widdowson MA
中科院分区：
文献类型：
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作者：
Ngere I;Munyua P;Harcourt J;Hunsperger E;Thornburg N;Muturi M;Osoro E;Gachohi J;Bodha B;Okotu B;Oyugi J;Jaoko W;Mwatondo A;Njenga K;Widdowson MA
尽管中东呼吸综合征冠状病毒（MERS-CoV）的高暴露，但在东非骆驼饲养实践中血清阳性的预测因素尚未得到很好的理解。我们进行了一项横断面调查，以描述骆驼群的概况，并确定与MERS冠状病毒血清阳性在北方肯尼亚的相关因素。我们招募了29个骆驼饲养户，并进行问卷调查，以收集牛群和家庭数据。从493只随机选择的骆驼中收集的血清样本使用微量中和试验检测抗MERS-CoV抗体，并使用回归分析将牛群和家庭特征与骆驼血清阳性相关联。家庭饲养骆驼（中位数= 23头骆驼，IQR 16-56），以及至少一个其他牲畜物种，分为两个不同的畜群;一个家庭畜群保持在家园附近，一个范围/fora畜群居住在远离宅基地。总体MERS-CoV IgG血清阳性率为76.3%，家庭和论坛牛群之间没有统计学显著差异。血清阳性的显著预测因子（P <0.05）包括6-10岁骆驼（aOR 2.3，95%CI 1.0-5.2）、2025头骆驼群（aOR 2.0，95%CI 1.2-3.4）和来自Gabra社区的骆驼（aOR 2.3，95%CI 1.2-4.2）。这些结果表明，病毒在骆驼中传播的程度很高，并有可能感染人类。
Despite high exposure to Middle East respiratory syndrome coronavirus (MERS-CoV), the predictors for seropositivity in the context of husbandry practices for camels in Eastern Africa are not well understood. We conducted a cross-sectional survey to describe the camel herd profile and determine the factors associated with MERS-CoV seropositivity in Northern Kenya. We enrolled 29 camel-owning households and administered questionnaires to collect herd and household data. Serum samples collected from 493 randomly selected camels were tested for anti-MERS-CoV antibodies using a microneutralisation assay, and regression analysis used to correlate herd and household characteristics with camel seropositivity. Households reared camels (median = 23 camels and IQR 16–56), and at least one other livestock species in two distinct herds; a home herd kept near homesteads, and a range/fora herd that resided far from the homestead. The overall MERS-CoV IgG seropositivity was 76.3%, with no statistically significant difference between home and fora herds. Significant predictors for seropositivity (P ⩽ 0.05) included camels 6–10 years old (aOR 2.3, 95% CI 1.0–5.2), herds with ⩾25 camels (aOR 2.0, 95% CI 1.2–3.4) and camels from Gabra community (aOR 2.3, 95% CI 1.2–4.2). These results suggest high levels of virus transmission among camels, with potential for human infection.