Screening for mineral oil saturated and aromatic hydrocarbons in paper and cardboard directly by planar solid phase extraction and by its coupling to gas chromatography
Screening for mineral oil saturated and aromatic hydrocarbons in paper and cardboard directly by planar solid phase extraction and by its coupling to gas chromatography
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DOI：
10.1016/j.chroma.2018.12.043
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发表时间：
2019-03-15
影响因子：
4.1
通讯作者：
Oellig, Claudia
中科院分区：
文献类型：
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作者：
Wagner, Melanie;Oellig, Claudia
矿物油饱和烃和芳香烃 (MOSH/MOAH) 是食品污染物，主要是由于再生纤维制成的包装材料中的迁移所致，但也已确定进入食品的其他途径。目前，食品和食品接触材料中 MOSH 和 MOAH 的法定限量尚未确定，但预计在不久的将来将会出台。对于 MOSH 和 MOAH 的分析，通常使用非常成熟且高度自动化的火焰离子化检测在线液相色谱-气相色谱 (LC-GC-FID)。然而，这种方法非常耗时，并且数据解释是一项非常具有挑战性的任务。因此，开发了一种平面固相萃取 (pSPE) 方法，用于快速有效地筛选纸张和纸板中的 MOSH 和 MOAH。 pSPE 基于高性能薄层色谱 (HPTLC)，可同时并行净化和分析多达 20 个样品，而 MOSH 和 MOAH 分析物经过双重开发后集中在硅胶 HPTLC 板上的两个不同目标区域。用报春碱浸渍板可以通过荧光检测 MOSH，而通过紫外光吸收检测 MOAH。 pSPE 筛选方法对 MOSH 和 MOAH 的检测限分别为 7.2 和 2.3 ng/区，相当于 1.8 和 0.6 mg/kg 试纸。 pSPE 与 GC 的耦合揭示了常见的 MOSH 和 MOAH 峰驼峰，而标记物质则提供了矿物油来源的鉴定或有关回收纤维材料的信息。与 SPE-GC-FID 分析相比，纸板样品中 MOSH 和 MOAH 的测定量表明 pSPE 是一种快速且合适的筛选工具。然而，聚烯烃低聚饱和烃、天然正烷烃、萜烯和可能的精油或甾醇酯的共迁移可能导致 MOSH 和 MOAH 的高估。因此，pSPE 结果高于给定限值的样品随后通过 GC (pSPE-GC) 进行分析，以进行确认和详细评估。 (C) 2018 Elsevier B.V. 保留所有权利。
Mineral oil saturated and aromatic hydrocarbons (MOSH/MOAH) are food contaminants, mainly due migration from packaging materials made from recycled fibers, but other routes of entry into food have also been identified. Legal limits for MOSH and MOAH in food and food contact materials currently are not set, but are to be expected in the near future. For the analysis of MOSH and MOAH, the very well developed and highly automated on-line liquid chromatography-gas chromatography with flame ionization detection (LC-GC-FID) is commonly used. However, this approach is time-consuming, and data interpretation is a very challenging task. Therefore, a planar solid phase extraction (pSPE) method was developed for a rapid and efficient MOSH and MOAH screening in paper and cardboard. Based on high-performance thin-layer chromatography (HPTLC), pSPE offers the simultaneous clean-up and analysis of up to 20 samples in parallel, while MOSH and MOAH analytes were focused in two distinct target zones on silica gel HPTLC plates after a twofold development. Plate impregnation with primuline allowed the detection of MOSH by fluorescence, while MOAH were detected by UV light absorption. The pSPE screening approach provided limits of detection of 7.2 and 2.3 ng/zone for MOSH and MOAH, respectively, corresponding to 1.8 and 0.6 mg/kg paper. Coupling of pSPE with GC revealed the common MOSH and MOAH peak humps, while marker substances offered the identification of the mineral oil origin or information about recycled fiber materials. As compared to SPE-GC-FID analyses, the determined quantities of MOSH and MOAH in cardboard samples indicated pSPE as a rapid and suitable screening tool. The co-migration of polyolefin oligomeric saturated hydrocarbons, native n-alkanes, terpenes and possibly essential oils or sterol esters, however, can result in an overestimation of MOSH and MOAH. Hence, samples with pSPE results above a given limit are subsequently analyzed by GC (pSPE-GC) for confirmation and detailed evaluation. (C) 2018 Elsevier B.V. All rights reserved.