COMPLETE CDNA AND DERIVED AMINO-ACID-SEQUENCE OF HUMAN FACTOR-V
COMPLETE CDNA AND DERIVED AMINO-ACID-SEQUENCE OF HUMAN FACTOR-V
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DOI：
10.1073/pnas.84.14.4846
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发表时间：
1987-07-01
影响因子：
11.1
通讯作者：
MANN, KG
中科院分区：
文献类型：
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作者：
JENNY, RJ;PITTMAN, DD;MANN, KG
已从用载体Charon 21 A制备的寡聚（dT）引发的人胎肝cDNA文库中分离出编码人因子V的cDNA克隆。来自三个重叠克隆的因子V的cDNA序列包括6672个碱基对（bp）的编码区、90个bp的5“非翻译区和163个bp的3”非翻译区，其中是poly（A）尾。推导的氨基酸序列由2224个氨基酸组成，包括28个氨基酸的前导肽。与人因子VIII的直接比较揭示了蛋白质之间在氨基酸序列和结构域结构上的相当大的同源性：三重A结构域和重复C结构域显示出与因子VIII中的相应结构域具有40%的同一性。如在因子VIII中，因子V的A结构域共享与铜蓝蛋白中的三个高度保守结构域具有40%的氨基酸序列同源性。因子V的B结构域含有35个串联和约9个额外的Asp-Leu-Ser-Gln-Thr-Thr/Asn-Leu-Ser-Pro形式的9个氨基酸的重复序列和2个额外的17个氨基酸的重复序列。因子V含有37个潜在的N-连接糖基化位点，其中25个在B结构域中，并且总共含有19个半胱氨酸残基。
cDNA clones encoding human factor v have been isolated from an oligo(dT)-primed human fetal liver cDNA library prepared with vector Charon 21A. The cDNA sequence of factor V from three overlapping clones includes a 6672-base-pair (bp) coding region, a 90-bp 5'' untranslated region, and a 163-bp 3'' untranslated region within which is a poly(A) tail. The deduced amino acid sequence consists of 2224 amino acids inclusive of a 28-amino acid leader peptide. Direct comparison with human factor VIII reveals considerable homology between proteins in amino acid sequence and domain structure: a tripicated A domain and duplicated C domain show .apprxeq. 40% identity with the corresponding domains in factor VIII. As in factor VIII, the A domains of factor V share .apprxeq. 40% amino acid-sequence homology with the three highly conserved domains in ceruloplasmin. The B domain of factor V contains 35 tandem and .apprxeq. 9 additional semiconserved repeats of nine amino acids of the form Asp-Leu-Ser-Gln-Thr-Thr/Asn-Leu-Ser-Pro and 2 additional semiconserved repeats of 17 amino acids. Factor V contains 37 potential N-linked glycosylation sites, 25 of which are in the B domain, and a total of 19 cysteine residues.