Atomic force microscopy analysis of normal and photoablated porcine corneas
Atomic force microscopy analysis of normal and photoablated porcine corneas
复制标题
DOI：
10.1016/j.jbiomech.2005.08.013
复制
发表时间：
2006-01-01
影响因子：
2.4
通讯作者：
Serrao, Sebastiano
中科院分区：
文献类型：
--
作者：
Lombardo, Marco;De Santo, Maria Penelope;Serrao, Sebastiano
我们展示了原子力显微镜（AFM）技术成像和分析角膜上皮和光消融角膜基质的能力和准确性。八个正常的猪角膜，其中一半使用扫描点准分子激光消融，进行了检查。所有角膜在戊二醛中固定后在平衡盐溶液中成像。在正常未处理的角膜中，我们观察到上皮表面显示出典型的多边形细胞，并呈现出许多微突起。作为前表面粗糙度测量的结果，将浅表上皮细胞分为三种类型。光消融角膜标本的AFM图像显示在消融基质表面上的起伏和颗粒状特征，特定于193nm ArF激光照射。然而，定量分析证实了准分子激光手术在去除亚微米组织方面的精确性。原子力显微镜是一种高分辨率的成像工具，用于扫描本地以及光消融角膜标本。此外，该技术允许在纳米尺度上对角膜平面进行精确的地形分析，其平滑度是重要的物理特性，并且是实现眼睛的最佳光学质量所必需的。(c)2005爱思唯尔有限公司保留所有权利。
We showed the capabilities and accuracy of atomic force microscopy (AFM) techniques for imaging and analyzing the corneal epithelium and the photoablated corneal stroma. Eight normal porcine corneas, half of which were ablated using a scanning-spot excimer laser, were examined. All the corneas were imaged in balanced salt solution after fixation in glutaraldehyde. In the normal untreated corneas we observed the epithelial surface showing the typical polygonal cells and presenting numerous microprojections. The superficial epithelial cells were classified in three types as a result of the anterior-surface roughness measurements. AFM images of the photoablated corneal specimens showed undulations and granule-like features on the ablated stromal surface, specific to 193nm ArF laser irradiation. Nevertheless, the quantitative analysis confirmed the precision of excimer laser surgery in removing submicrometric amounts of tissue. AFM showed to be a high-resolved imaging tool for the scanning of both native as well as photoablated corneal specimens. Also, this technique permits precise topographic analysis of the corneal plane, in the nanometric scale, of which smoothness is an important physical characteristic and necessary to achieve an optimal optical quality of the eye. (c) 2005 Elsevier Ltd. All rights reserved.