Predicting Genes in Single Genomes with AUGUSTUS.
Predicting Genes in Single Genomes with AUGUSTUS.
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DOI：
10.1002/cpbi.57
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发表时间：
2019-03-01
影响因子：
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通讯作者：
Stanke, Mario
中科院分区：
文献类型：
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作者：
Hoff, Katharina J;Stanke, Mario
AUGUSTUS 是一种在基因组序列中寻找蛋白质编码基因及其外显子-内含子结构的工具。它不一定需要额外的实验输入，因为它可以以所谓的从头开始模式应用。然而，可以整合来自各种来源的外在证据，例如转录组测序或密切相关的基因组的注释，以提高注释的准确性和完整性。 AUGUSTUS 可以应用于单个基因组，或同时应用于多个对齐的基因组。在这里，我们描述了训练 AUGUSTUS 进行个体基因组注释所需的步骤以及进行实际结构注释的步骤。此外，我们描述了来自各种外部证据来源的证据的生成和整合。 © 2018 John Wiley & Sons, Inc. 版权所有
AUGUSTUS is a tool for finding protein-coding genes and their exon-intron structure in genomic sequences. It does not necessarily require additional experimental input, as it can be applied in so-called ab initio mode. However, extrinsic evidence from various sources such as transcriptome sequencing or the annotations of closely related genomes can be integrated in order to improve the accuracy and completeness of the annotation. AUGUSTUS can be applied to single genomes, or simultaneously to several aligned genomes. Here, we describe steps required for training AUGUSTUS for the annotation of individual genomes and the steps to do the actual structural annotation. Further, we describe the generation and integration of evidence from various sources of extrinsic evidence. © 2018 by John Wiley & Sons, Inc.