Metabolism of very-low-density lipoprotein and low-density lipoprotein containing apolipoprotein C-III and not other small apolipoproteins.
Metabolism of very-low-density lipoprotein and low-density lipoprotein containing apolipoprotein C-III and not other small apolipoproteins.
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DOI：
10.1161/atvbaha.109.197830
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发表时间：
2010-02
期刊：
影响因子：
--
通讯作者：
Sacks FM
中科院分区：
文献类型：
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作者：
Mendivil CO;Zheng C;Furtado J;Lel J;Sacks FM
我们旨在阐明载脂蛋白C-III （apoCIII）对人载脂蛋白B代谢的影响。我们研究了四种VLDL， IDL和LDL类型的动力学：OtherApos−CIII−：无apoCIII， apoAII, apoi， apoCII或apoE；OtherApos+CIII−：无apoCIII，但至少有一种；OtherApos−CIII+: apoCIII，但不包括其他；OtherApos+CIII+: apoCIII和至少一个其他。VLDL和IDL OtherApos−CIII+和OtherApos−CIII−具有相似的脂溶转化为更小颗粒的速率。然而，轻LDL OtherApos−CIII+与OtherApos−CIII−相比，转化为致密LDL的速度要快得多，轻LDL OtherApos+CIII+与OtherApos+CIII−相比也是如此。VLDL和IDL OtherApos+CIII +在循环中的直接清除最少，而富含apoE的VLDL和IDL OtherApos+CIII−的清除速度很快。OtherApos+CIII+也富含apoE的部分脂蛋白清除率非常低。结果表明，apoCIII强烈抑制VLDL和IDL的肝脏摄取，当两者存在时，apoCIII的相反影响高于apoE。apoCIII在高密度VLDL上的存在与向IDL的缓慢转化（一种脂蛋白脂肪酶依赖的过程）无关；但在低密度脂蛋白时，apoCIII与向高密度LDL的转化增强有关，这一过程涉及肝脂肪酶。
We aimed to clarify the influence of apolipoprotein C-III (apoCIII) on human apolipoprotein B metabolism. We studied the kinetics of four VLDL, IDL and LDL types containing: OtherApos−CIII−: none of apoCIII, apoAII, apoCI, apoCII or apoE; OtherApos+CIII−: no apoCIII but at least one of the others; OtherApos−CIII+: apoCIII, but not any others; OtherApos+CIII+: apoCIII and at least one other. VLDL and IDL OtherApos−CIII+ and OtherApos−CIII− had similar rates of lipolytic conversion to smaller particles. However, light LDL OtherApos−CIII+ compared to OtherApos−CIII− had much faster conversion to dense LDL, as did light LDL OtherApos+CIII+ compared to OtherApos+CIII−. VLDL and IDL OtherApos−CIII+ had minimal direct removal from circulation, while VLDL and IDL OtherApos+CIII−, rich in apoE, showed fast clearance. Lipoproteins in fraction OtherApos+CIII+ also rich in apoE had very low clearance. The results suggest that apoCIII strongly inhibits hepatic uptake of VLDL and IDL overriding the opposite influence of apoE when both are present. The presence of apoCIII on dense VLDL is not associated with slow conversion to IDL, a lipoprotein lipase dependent process; but when on light LDL apoCIII is associated with enhanced conversion to dense LDL, a process involving hepatic lipase.