ENDOTOXIN-INDUCED UVEITIS IN RATS AS A MODEL FOR HUMAN-DISEASE
ENDOTOXIN-INDUCED UVEITIS IN RATS AS A MODEL FOR HUMAN-DISEASE
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DOI：
10.1038/286611a0
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发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
EGBERT, PR
中科院分区：
文献类型：
--
作者：
ROSENBAUM, JT;MCDEVITT, HO;EGBERT, PR
急性前葡萄膜炎是一种病因不明的较常见疾病。它与多种全身性疾病的关联，包括强直性脊柱炎、Reiter综合征、behet病、幼年类风湿性关节炎、银屑病关节炎、炎症性肠病和结节病，以及HLA抗原B27在无相关疾病病例中的流行（参考文献1），已被注意到，但尚未解释。总的来说，葡萄膜炎的动物模型尚未阐明人类疾病的机制。大多数需要直接玻璃体内注射或用视网膜或葡萄膜抗原反复免疫。葡萄膜炎确实发生在佐剂性关节炎中，这种综合征可在大鼠中通过全身注射多种革兰氏阳性细菌细胞壁的肽-狗聚糖而产生。这种疾病最初是在矿物油中注射分枝杆菌后被发现的，它与瑞特综合征有许多相似之处，包括在多达40%的动物身上出现的眼部病变。由于瑞特氏综合征与肠内革兰氏阴性菌感染有关，如阿希菌、沙门氏菌和耶尔森菌，我们试图通过注射杀死的革兰氏阴性菌在大鼠中产生关节炎。虽然我们没有在这些动物中观察到临床关节炎，但仔细的组织学研究表明葡萄膜炎几乎是普遍存在的。下面的研究表明，眼病是由于被称为内毒素或脂多糖（LPS）的细菌细胞壁的一部分。
Acute anterior uveitis is a relatively common disease of uncertain aetiology. Its association with a wide variety of systemic disorders including ankylosing spondylitis, Reiter's syndrome, Behçet's disease, juvenile rheumatoid arthritis, psoriatic arthritis, inflammatory bowel disease and sarcoidosis and the prevalence of the HLA antigen B27 (ref. 1) in cases without associated disease have been noted but not explained. In general, animal models for uveitis have not clarified the mechanism of human disease. Most require direct intravitreal injection2or repeated immunizations with retinal or uveal antigens3. Uveitis does occur in adjuvant arthritis, a syndrome that can be produced in rats by the systemic injection of pepti-doglycan from the cell wall of a variety of Gram-positive bacteria4. The disease, which was initially described following the injection of mycobacteria in mineral oil, has many similarities to Reiter's syndrome5, including the development of eye pathology in as many as 40% of the animals6. As Reiter's syndrome is associated with enteric Gram-negative infections such asShigella,SalmonellaandYersinia, we sought to produce an arthritis in rats by injecting killed Gram-negative bacteria. Although we have not observed clinical arthritis in these animals, careful histological study has shown the near-universal appearance of uveitis. The studies described below indicate that the eye disease is due to a portion of the bacterial cell wall known as endotoxin or lipopolysaccharide (LPS).