Construction of high-throughput genotyped chromosome segment substitution lines in rice (Oryza sativa L.) and QTL mapping for heading date
Construction of high-throughput genotyped chromosome segment substitution lines in rice (Oryza sativa L.) and QTL mapping for heading date
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水稻高通量基因型染色体片段替换系的构建及抽穗期QTL定位
DOI：
10.1111/pbr.12248
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发表时间：
2015
期刊：
影响因子：
2
通讯作者：
Liang Guohua
中科院分区：
文献类型：
--
作者：
Zhu Jinyan;Niu Yongchao;Tao Yajun;Wang Jun;Jian Jianbo;Tai Shuaishuai;Li Jun;Yang Jie;Zhong Weigong;Zhou Yong;Liang Guohua
染色体片段代换系为复杂性状的数量性状位点定位和遗传解剖提供了理想的材料。在本研究中，我们建立了一套由175个系组成的CSSL群体，该群体是由受体‘广爱4号’和供体‘日本裸’衍生而来的。基于260个分子标记，我们首先构建了核心97个品系的物理图谱。然后，根据重测序数据对这97个品系进行基因分型，并构建基于重测序的物理图谱。与基于分子标记的物理图谱相比，基于重测序的物理图谱包含367个替代片段和252个新发现片段。367个取代片段的总大小为1074 Mb，是水稻基因组大小的2.81倍。以97个cssl为材料，鉴定出9个与抽穗期有关的qtl，其中3个为首次报道。所有qtl加性效应均为正，加性效应范围为9.50 ~ 16.50 d。这些CSSLs可为水稻功能基因组学研究和分子育种提供新的资源。
Chromosome segment substitution lines (CSSLs) provide ideal materials for quantitative trait loci (QTLs) mapping and genetic dissection of complex traits. In this study, we developed a set of CSSL population consisting of 175 lines, which were derived between the recipient ‘Guangluai 4’ and the donor ‘Nipponbare’. Based on 260 molecular markers, we firstly constructed a physical map of core 97 lines. Then, these 97 lines were further genotyped based on resequencing data, and a resequencing‐based physical map was constructed. Compared with the molecular marker‐based physical map, the resequencing‐based physical map of 97 lines contained 367 substituted segments with 252 newly discovered segments. The total size of the 367 substituted segments was 1,074 Mb, which was 2.81 times the size of rice genome. Using the 97 CSSLs as materials, we identified nine QTLs for heading date and three of them were firstly reported. All the QTLs had positive additive effects, ranging from 9.50 to 16.50 days. These CSSLs may greatly help forge a new resource for functional genomics studies and molecular breeding in rice.