Induction of a polyubiquitin gene (ubi1) by potato phytoalexins and heat shock in Gibberella pulicaris
Induction of a polyubiquitin gene (ubi1) by potato phytoalexins and heat shock in Gibberella pulicaris
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DOI：
10.1007/s002940050414
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发表时间：
1998-12-01
期刊：
影响因子：
2.5
通讯作者：
Weltring, KM
中科院分区：
文献类型：
--
作者：
Loser, K;Weltring, KM
马铃薯干腐病的病原菌是赤霉菌，它通过伤口感染马铃薯块茎，在那里它会接触到植物抗菌素rishitin？还有卢比明。菌丝体孵化还是？添加植物保健素的琼脂瞬间诱导了一个多泛素基因的转录，该基因由四个泛素单位组成，从头到尾排列；：第四个单位包含一个54bp？内含子和编码蛋白的另一个谷氨酰胺，在G基因组的C末端，揭示了分离的多泛素基因的一个拷贝和其他泛素基因的一个或两个拷贝，在100微克/毫升的里希丁的阈值以上和在高温下，可以检测到该基因转录的增加，这表明暴露于植物保卫素会引起与热休克后相似的菌丝细胞的应激反应。
Gibberella pulicaris, a causal agent of potato dry rot, infects potato tubers via wounds, where it is exposed to the phytoalexins rishitin? and lubimin. Incubation of mycelium or? agar supplemented with phytoalexins transiently induced the transcription of a polyubiquitin gene consisting of four ubiquitin units arranged head to tail;:he fourth unit contains a 54-bp? intron and an additional glutamine of the encoded protein, Southern at the C-terminus of the G. pulicaris genome revealed one copy of the isolated polyubiquitin gene and one or two copies of other ubiquitin genes, Increased transcription of the gene was detectable above a threshold of 100 mu g/ml of rishitin and at elevated temperatures, which; indicates that exposure to phytoalexins causes a stress reaction of hyphal cells similar to that after heat shock.