Fibroblast growth factor receptor 2 plays an essential role in telencephalic progenitors
Fibroblast growth factor receptor 2 plays an essential role in telencephalic progenitors
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DOI：
10.1159/000112521
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Gaiano, Nicholas
中科院分区：
文献类型：
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作者：
Ever, Leah;Zhao, Rui-jing;Gaiano, Nicholas
我们使用功能丧失分析来确定成纤维细胞生长因子受体 2 (FGFR2) 在端脑祖细胞中的作用，并检查 FGFR 和 Notch 信号传导之间的相互作用。虽然 FGFR2 突变体的端脑看起来非常正常，但突变体端脑祖细胞在体内和体外表现出改变的增殖行为。根据我们之前的发现，Notch1 激活增加了 FGF2 中的神经球频率，我们测试了这种效应是由 FGFR1 还是 FGFR2 介导的。我们发现Notch1激活增加了FGFR1或FGFR2突变细胞中神经球的频率，但对这些受体突变的神经球大小的减小没有影响。其他分析揭示了 FGFR2 IIIc 亚型的成人新皮质突变体的生化变化，以及 FGFR2 在鼻咽、眼睑和角膜发育中的重要作用。版权所有 (C) 2007 S. Karger AG，巴塞尔。
We used loss-of-function analysis to determine the role of fibroblast growth factor receptor 2 (FGFR2) in telencephalic progenitors, and also to examine interactions between FGFR and Notch signaling. While the telencephalon of FGFR2 mutants appears grossly normal, mutant telencephalic progenitors exhibit altered proliferative behavior in vivo and in vitro. Based upon our prior finding that Notch1 activation increased neurosphere frequency in FGF2, we tested whether this effect is mediated by FGFR1 or FGFR2. We found that Notch1 activation increased neurosphere frequency in cells mutant for either FGFR1 or FGFR2, but had no effect on the reduced size of neurospheres mutant for those receptors. Additional analyses revealed biochemical changes in the adult neocortex mutant for the IIIc isoform of FGFR2, and essential roles for FGFR2 in nasopharynx, eyelid, and cornea development. Copyright (C) 2007 S. Karger AG, Basel.