Isolation and Identification of Antialgal Compounds from the Leaves of Vallisneria spiralis L. by Activity-Guided Fractionation (5 pp)
Isolation and Identification of Antialgal Compounds from the Leaves of Vallisneria spiralis L. by Activity-Guided Fractionation (5 pp)
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DOI：
10.1065/espr2006.06.314
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发表时间：
2006-06
影响因子：
5.8
通讯作者：
Qiming Xian;Haidong Chen;Huili Liu;H. Zou;Daqiang Yin
中科院分区：
文献类型：
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作者：
Qiming Xian;Haidong Chen;Huili Liu;H. Zou;Daqiang Yin
苦草是一种常见的沉水植物，广泛存在于东南亚的湖泊、池塘、沼泽和溪流的平静水域中。螺旋藻不仅在减少水体富营养化方面具有重要的生产力，而且在抑制蓝藻生长方面也发挥着重要作用。采用活性导向分离和柱色谱分离鉴定螺旋藻提取物中的化感物质。方法螺旋藻叶片经洗涤、风干、95%乙醇回流提取。采用柱层析和分级分离的方法分离得到具有抗藻活性的提取物。结果采用柱层析和活性导向分级分离的方法从螺旋藻提取物中分离得到2个具有较强抑藻活性的组分。在第一组分中鉴定出2-乙基-3-甲基马来酰亚胺、二氢丙烯内酯和4-氧代-β-紫罗兰酮，在第二组分中鉴定出3-羟基-5，6-环氧基-β-紫罗兰酮、Loliolide、6-羟基-3-氧代-α-紫罗兰酮和一个未知化合物。它们对铜绿微囊藻Kütz有较强的抑制作用。讨论2-乙基-3-甲基马来酰亚胺是叶绿素光氧化的副产物，另有5个化合物为β-胡萝卜素的衍生物。采用高分辨气相色谱-质谱法和衍生化技术对其分子结构进行鉴定和确认。未知化合物的分子式为C16H19NO4。结论从螺旋藻中鉴定出6种化合物，其中2-乙基-3-甲基马来酰亚胺是主要的化感物质，紫罗兰酮类化合物也是潜在的化感物质。我们的研究结果有助于我们进一步研究藻类抑制作用的机理，开发新的潜在的抗藻类物质。
Background, Aims and ScopeVallisneria spiralis Linn., a common, submerged macrophyte, is widely available in quiet waters of lakes, ponds, marshes and streams in Southeast Asia. V. spiralis plays a significant role not only in decreasing eutrophication of water body for its productivity, but also in inhibiting the growth of blue-green algae? The aim of the paper involves the isolation and identification of allelochemicals from extracts of V. spiralis by activity-guided fractionation and column chromatography.MethodsLeaves of V. spiralis was washed free of debris, air-dried and refluxed in 95% EtOH. The extract was isolated using column chromatography and fractionation with antialgal activity. Potential allelochemicals were analyzed by high-resolution gas chromatography-mass spectrometry (HRGC-MS).ResultsTwo fractions with strong antialgal activity were isolated using column chromatography and activity-guided fractionation from the extract of V. spiralis. 2-Ethyl-3-methylmaleimide, dihydroactinidiolide and 4-oxo-β-Ionone were identified in the first fraction, and 3-hydroxy-5,6-epoxy-β-ionone, loliolide, 6-hydroxy-3-oxo-α-ionone and an unknown compound in the second fraction. They had strong inhibitory effects on Microcystis aeruginosa Kütz.Discussion2-Ethyl-3-methylmaleimide is a byproduct of photooxidation of chlorophyll, and five other compounds identified were derivatives of β-carotene. HRGC-MS and derivatization technology were used to identify and confirm their molecular structures. The formula of the unknown compound was C16H19NO4. Metabolites of plant pigments had strong inhibitory activities on growth of algae.ConclusionsSix compounds had been identified in V. spiralis, among them, 2-ethyl-3-methylmaleimide was the main allelochemical, and derivatives of ionone were also potential allelochemicals.Recommendations and Perspective. The results of our research could help us to study further mechanisms of inhibitory effect on algae and develop new potential antialgal substances.