Niche adaptation by expansion and reprogramming of general transcription factors.
Niche adaptation by expansion and reprogramming of general transcription factors.
复制标题
通过一般转录因子的扩展和重编程进行生态位适应。
DOI：
10.1038/msb.2011.87
复制
发表时间：
2011
影响因子：
9.9
通讯作者：
Baliga,NitinS
中科院分区：
文献类型：
--
作者：
Turkarslan,Serdar;Reiss,DavidJ;Gibbins,Goodwin;Su,WanLin;Pan,Min;Bare,JChristopher;Plaisier,ChristopherL;Baliga,NitinS
古细菌中转录因子B（TFB）家族的许多谱系特异性扩增表明扩增的TFB在编码环境特异性基因调控程序中发挥重要作用。鉴于高盐度湖泊的特点，嗜盐古菌中异常大量的TFB进一步表明，它们在快速适应动态变化环境的挑战方面可能特别重要。受这些观察结果的启发，我们通过将盐生盐杆菌NRC-1中所有7个TFB的序列变异、调节和物理相互作用与它们的适应性景观、功能层次和遗传相互作用相关联，研究了TFB扩增的影响，这些实验涵盖了盐、pH、温度和Cu胁迫的组合变化。这项系统分析揭示了一个优雅的方案，其中基因转换事件产生了全新的适应性景观，这些事件对重复的TFB的调节或物理相互作用产生了微妙的变化。基于这些见解，我们引入了一种综合重新设计的TFB，并改变了现有TFB的调控，以说明古细菌如何通过简单地重新编程其TFB调控网络来快速产生新的表型。
Numerous lineage‐specific expansions of the transcription factor B (TFB) family in archaea suggests an important role for expanded TFBs in encoding environment‐specific gene regulatory programs. Given the characteristics of hypersaline lakes, the unusually large numbers of TFBs in halophilic archaea further suggests that they might be especially important in rapid adaptation to the challenges of a dynamically changing environment. Motivated by these observations, we have investigated the implications of TFB expansions by correlating sequence variations, regulation, and physical interactions of all seven TFBs inHalobacterium salinarumNRC‐1 to their fitness landscapes, functional hierarchies, and genetic interactions across 2488 experiments covering combinatorial variations in salt, pH, temperature, and Cu stress. This systems analysis has revealed an elegant scheme in which completely novel fitness landscapes are generated by gene conversion events that introduce subtle changes to the regulation or physical interactions of duplicated TFBs. Based on these insights, we have introduced a synthetically redesigned TFB and altered the regulation of existing TFBs to illustrate how archaea can rapidly generate novel phenotypes by simply reprogramming their TFB regulatory network.