Collagen vitrigel membrane useful for paracrine assays in vitro and drug delivery systems in vivo
Collagen vitrigel membrane useful for paracrine assays in vitro and drug delivery systems in vivo
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DOI：
10.1016/j.jbiotec.2007.05.033
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发表时间：
2007-08-01
影响因子：
4.1
通讯作者：
Enosawa, Shin
中科院分区：
文献类型：
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作者：
Takezawa, Toshiaki;Takeuchi, Tomoyo;Enosawa, Shin
我们之前利用热变性蛋白玻璃化的概念，成功地将一种软而浑浊的i型胶原凝胶盘转化为一种强而透明的玻璃膜，并证明了它的蛋白质渗透性和作为重建两种不同细胞类型之间的串扰模型的支架的优势。在这项研究中，我们观察了i型胶原玻璃体膜的纳米结构，并验证了其在体外旁分泌分析和体内给药系统中的实用性。扫描电镜观察显示，玻璃体膜为典型i型胶原原纤维的致密网状结构。在PC-12嗜铬细胞与L929成纤维细胞间的串扰模型中，L929细胞分泌的神经生长因子（NGF）通过胶原玻璃体膜，通过旁分泌作用诱导PC-12细胞突突生长。含有血管内皮生长因子（VEGF）的胶原玻璃体膜在体外显示出VEGF的缓释，并在大鼠皮下移植后产生了显著的血管新生。这些数据表明，胶原玻璃体膜是有用的旁分泌分析在体外和药物输送系统在体内。(c) 2007 Elsevier B.V.版权所有
We previously succeeded in converting a soft and turbid disk of type-I collagen gel into a strong and transparent vitrigel membrane utilizing a concept for the vitrification of heat-denatured proteins and have demonstrated its protein-permeability and advantage as a scaffold for reconstructing crosstalk models between two different cell types. In this study, we observed the nano-structure of the type-I collagen vitrigel membrane and verified its utility for paracrine assays in vitro and drug delivery systems in vivo. Scanning electron microscopic observation revealed that the vitrigel membrane was a dense network architecture of typical type-I collagen fibrils. In the crosstalk model between PC-12 pheochromocytorna cells and L929 fibroblasts, nerve growth factor (NGF) secreted from L929 cells passed through the collagen vitrigel membrane and induced the neurite outgrowth of PC-12 cells by its paracrine effect. Also, the collagen vitrigel membrane containing vascular endothelial growth factor (VEGF) showed sustained-release of VEGF in vitro and its subcutaneous transplantation into a rat resulted in remarkable angiogenesis. These data suggest that the collagen vitrigel membrane is useful for paracrine assays in vitro and drug delivery systems in vivo. (c) 2007 Elsevier B.V. All rights reserved.