Inactivation of human norovirus by chlorous acid water, a novel chlorine-based disinfectant
Inactivation of human norovirus by chlorous acid water, a novel chlorine-based disinfectant
复制标题
DOI：
10.1016/j.jiac.2021.10.001
复制
发表时间：
2021-11-14
影响因子：
2.2
通讯作者：
Kuwahara, Tomomi
中科院分区：
文献类型：
--
作者：
Goda, Hisataka;Nakayama-Imaohji, Haruyuki;Kuwahara, Tomomi
前言：人类诺如病毒(HuNoV)是感染性胃肠炎的主要病原。由于胡诺夫病毒对酒精具有抵抗力，因此使用氯基消毒剂来净化环境表面的病毒。氯酸水(CA)最近被日本批准为新型氯基消毒剂，被归类为非处方药2类。在本研究中，我们旨在评价CA灭活HuNoV的能力。方法：用CA和次氯酸钠(NaClO)作为实验消毒剂，用逆转录定量聚合酶链式反应(RT-qPCR)检测核糖核酸酶(RNase)处理后的残留RNA拷贝数。此外，比较了在牛血清白蛋白(BSA)、绵羊红细胞(SRBC)、多肽、肉类提取物或氨基酸存在下，CA和NaClO对HuNoV RNA拷贝数的log10减少，以评价这些消毒剂在富含有机物的条件下的稳定性。结果：在不含有机物的情况下，提供200ppm游离有效氯的CA在5分钟内使HuNoV RNA拷贝数减少3.0log10。即使在高有机质负荷下(BSA和SRBC各0.3%或0.5%蛋白胨)，200ppm CA仍能使HuNoV RNA拷贝数减少3.0log10，而1000ppm次氯酸钠(NaClO)在0.5%蛋白胨存在下可使HuNoV RNA拷贝数减少不到1.0log10。CA只与半胱氨酸、组氨酸和谷胱甘肽反应，而NaClO与所有氨基酸反应。结论，CA是富含有机物条件下灭活虎新病毒的有效消毒剂。
Introduction: Human norovirus (HuNoV) is a leading cause of infectious gastroenteritis. Since HuNoV shows resistance to alcohol, chlorine-based sanitizers are applied to decontaminate the virus on environmental surfaces. Chlorous acid water (CA) has been recently approved as a novel chlorine-based disinfectant categorized as a Type 2 OTC medicine in Japan. In this study, we aimed to evaluate the capability of CA to inactivate HuNoV. Methods: HuNoV (genogroups GII.2 and GII.4) was exposed to the test disinfectants including CA and sodium hypochlorite (NaClO), and the residual RNA copy was measured by reverse transcription quantitative PCR (RTqPCR) after pretreatment with RNase. In addition, the log10 reduction of HuNoV RNA copy number by CA and NaClO was compared in the presence of bovine serum albumin (BSA), sheep red blood cells (SRBC), polypeptone, meat extract or amino acids to evaluate the stability of these disinfectants under organic-matter-rich conditions. Results: In the absence of organic substances, CA with 200 ppm free available chlorine provided >3.0 log10 reduction in the HuNoV RNA copy number within 5 min. Even under high organic matter load (0.3% each of BSA and SRBC or 0.5% polypeptone), 200 ppm CA achieved >3.0 log10 reduction in HuNoV RNA copy number while less than 1.0 log10 reduction was observed with 1,000 ppm sodium hypochlorite (NaClO) in the presence of 0.5% polypeptone. CA reacted with only cysteine, histidine and glutathione while NaClO reacted with all of the amino acids tested. Conclusions: CA is an effective disinfectant to inactivate HuNoV under organic-matter-rich conditions.