Physicochemical properties and structural characterization of a galactomannan from Sophora alopecuroides L. seeds
Physicochemical properties and structural characterization of a galactomannan from Sophora alopecuroides L. seeds
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苦豆子半乳甘露聚糖的理化性质和结构表征
DOI：
10.1016/j.carbpol.2015.12.058
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发表时间：
2016-04-20
影响因子：
11.2
通讯作者：
Sun, Xiangjun
中科院分区：
文献类型：
--
作者：
Guo, Rui;Ai, Lianzhong;Sun, Xiangjun
从苦豆子粗多糖中分离得到一种水溶性中性多糖(F25)。对其物化性质和结构进行了研究。I-2-KI和刚果红反应表明，F25含有许多无三螺旋构象的支链，这与甲基化和核磁共振分析相吻合，即F25是一种半乳甘露聚糖，其主链为1A连接的β-D-MANP(M/G1.48-1.84)，主要由T-CEAO-Galp(86.82%)或少量1，4-CEAO-Galp(13.18%)在0-6处随机分布。与去离子水的[ETA](2.98 dL/g)相比，NaCl(2.89-2.90 dL/g)和NaOH(2.27-2.28 dL/g)可以影响F25在溶液中的本征相互作用和构象，导致分子间的非缠结和分子内缔合。这一点通过三维分子建模得到了验证。此外，与其他多糖相比，F25具有更高的结晶度(25.7%)和更大的微晶尺寸(7.6 nm)。本研究获得了F25的分子结构与理化性质之间的可能关系，为其在食品和医药行业中的应用奠定了基础。(C)2015爱思唯尔有限公司。保留所有权利。
A water-soluble neutral polysaccharide (F25) was fractionated from crude polysaccharides of Sophora alopecuroides L. seeds. Its physicochemical properties and structural characterization were investigated. The I-2-KI and Congo red reactions showed F25 possessed many branches without triple -helical conformation, which coincided with methylation and NMR analyses, i.e., F25 was a galactomannan with 1A-linked beta-D-Manp backbone distributed randomly (M/G 1.48-1.84) by major T-ceao-Galp (86.82%) or minor 1,4 linked ceao-Galp (13.18%) at 0-6. Compared with [eta] of deionized water (2.98 dL/g), it was indicated that NaC1 (2.89-2.90 dL/g) and NaOH (2.27-2.28 dL/g) could affect intrinsic interactions and conformation of F25 in solution, leading to molecular non-entanglements and intramolecular associations. This was demonstrated by 3D molecular modeling. Furthermore, the amorphous nature of F25 was revealed with higher crystallinity (25.7%) and crystallite size (7.6 nm) compared to other polysaccharides. In this study, the possible relationships between molecular structure and physicochemical properties were obtained for F25, which could be potentially applied in food and pharmaceutical industries. (C) 2015 Elsevier Ltd. All rights reserved.