Determination of methylcellulose and hydroxypropyl methylcellulose food gums in food and food products: collaborative study.
Determination of methylcellulose and hydroxypropyl methylcellulose food gums in food and food products: collaborative study.
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食品和食品中甲基纤维素和羟丙基甲基纤维素食品胶的测定：合作研究。
DOI：
10.1093/jaoac/90.3.786
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发表时间：
2007
影响因子：
1.6
通讯作者：
Stephanie K. Lynch
中科院分区：
文献类型：
--
作者：
R. Harfmann;B. Deshmukh;J. Conklin;M. Turowski;Stephanie K. Lynch
对食品中甲基纤维素（MC）和羟丙基甲基纤维素（HPMC）测定方法的重现性进行了研究。这些广泛使用的食品胶具有不寻常的溶解度特征，无法通过现有的膳食纤维方法准确测定。新方法使用AOAC官方方法991.43的酶消化程序。消化液必须冷藏以使MC或HPMC完全水合。过滤冷却的溶液并通过尺寸排阻液相色谱法进行分析。合作实验室收到了28份含有0- 100% MC或HPMC的样品。样本集包括5种食物基质（面包、牛奶、鱼、马铃薯和粉状果汁饮料）的盲法重复。Cochran和Grubbs检验用于消除离群值。对于含有MC的食品样品，实验室内精密度，重复性相对标准偏差（RSDr）的值，范围从4.2%至16%，和值的重复性实验室精密度，再现性相对标准偏差（RSDR），范围从11%至20%。对于HPMC样品，RSDr值范围为6.4 - 27%，RSDR值范围为17 - 39%。食品基质中MC和HPMC的回收率范围为78 - 101%。这些结果表明，MC和HPMC测定的精密度和重现性可接受，不存在官方AOAC方法。建议将此方法作为AOAC官方第一行动。
A collaborative study was performed to determine the reproducibility of a method for the determination of methylcellulose (MC) and hydroxypropyl methylcellulose (HPMC) in food. These widely used food gums possess unusual solubility characteristics and cannot accurately be determined by existing dietary fiber methods. The new method uses the enzyme-digestion procedure of AOAC Official Method 991.43. Digestate solutions must be refrigerated to fully hydrate MC or HPMC. The chilled solutions are filtered and analyzed by size-exclusion liquid chromatography. Collaborating laboratories received 28 samples containing MC or HPMC in the range of 0-100%. The sample set included blind duplicates of 5 food matrixes (bread, milk, fish, potato, and powdered juice drink). Cochran and Grubbs tests were used to eliminate outliers. For food samples containing MC, values for within-laboratory precision, repeatability relative standard deviation (RSDr), ranged from 4.2 to 16%, and values for among-laboratories precision, reproducibility relative standard deviation (RSDR), ranged from 11 to 20%. For HPMC samples, RSDr values ranged from 6.4 to 27%, and RSDR values ranged from 17 to 39%. Recoveries of MC and HPMC from the food matrixes ranged from 78 to 101%. These results show acceptable precision and reproducibility for the determination of MC and HPMC, for which no Official AOAC Methods exist. It is recommended that this method be adopted as AOAC Official First Action.