Potential of Next-generation POCT in Infectious Disease Rapid Test.
Potential of Next-generation POCT in Infectious Disease Rapid Test.
复制标题
DOI：
10.3314/mmj.17.012
复制
发表时间：
2017-01-01
影响因子：
1
通讯作者：
Shimetani, Naoto
中科院分区：
文献类型：
--
作者：
Shimetani, Naoto
在医疗实践中，实时检测结果对于早期诊断和确定治疗方向是必要的。护理点检测(POCT)是一种通过床边实时检测为诊断和治疗提供有益和有用信息的检测系统。因此，POCT在传染病领域具有很高的实用价值，因为它是一种快速检测方法，可以在会诊时间内为初步治疗提供有用的信息。传染病快速检测试剂盒可用于各种预见性病原体靶标，包括细菌、病毒、真菌、原虫和其他疾病制剂。其中一种试剂盒是免疫层析分析(ICA)，这是一种用于POCT的测量方法，操作简单，甚至医生和护士都可以进行测试。血清诊断方法已在日本的医疗实践中辅助使用，用于深部真菌病的早期临床诊断，作为确定治疗方案的一种手段。然而，这种方法很复杂，被认为是一种全面的临床检查；因此，它不包括在POCT的范畴内。最近，环路介导的等温扩增(LAMP)方法在美国被测试为一种诊断真菌感染的POCT方法。在不久的将来，有望开发一种使用简单和高精度POCT的实验室程序来早期诊断深部真菌病。
Real-time test results are necessary for early diagnosis and in determining treatment orientation in medical practice. Point of Care Testing (POCT) is a testing system that provides beneficial and helpful information for diagnosis and treatment through real-time testing at the bedside. Therefore, POCT has high utility value in the field of infectious diseases as a rapid test that provides, within the consultation hours, useful information for initial treatment.Infectious disease rapid test kits are commercially available for a wide variety of prophlogistic pathogen targets, including bacterial, viral, fungal, protozoal, and other disease agents. One of these kits is immunochromatography assay (ICA), a measuring method used as POCT that is easy to operate, wherein even physicians and nurses can conduct the test.Serodiagnostic method has been adjunctively used in medical practice in Japan for early clinical diagnosis of deep mycosis as a means to determine treatments. However, this method is complicated and is considered a full-scale clinical examination; therefore, it is not included in the category of POCT. Recently, Loop-Mediated Isothermal Amplification (LAMP) method has been tested as a POCT for diagnosis of fungal infection in the US. Development of a laboratory procedure using simple and highly accurate POCT for early diagnosis of deep mycosis is expected in the near future.