Quantitative imaging of microvascular blood flow networks in deep cortical layers by 1310 nm μODT.
Quantitative imaging of microvascular blood flow networks in deep cortical layers by 1310 nm μODT.
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DOI：
10.1364/ol.40.004293
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发表时间：
2015-09-15
期刊：
影响因子：
3.6
通讯作者：
Pan Y
中科院分区：
文献类型：
--
作者：
You J;Zhang Q;Park K;Du C;Pan Y
人们对新的神经图像技术的兴趣越来越大，这种技术不仅可以对毛细血管中的脑血流速度（CBFv）进行高分辨率量化，而且还可以在大范围内绘制CBFv网络动态。这种成像能力对于解码刺激下不同皮质层的功能差异具有重要意义。为了解决光学穿透深度的限制，我们提出了一种新的1310 nm的超高分辨率光学相干多普勒断层扫描（μ ODT）系统，并将其与先前的800 nm μ ODT系统进行比较，用于小鼠脑三维CBFv成像。我们表明，新的1310 nmµODT允许定量CBFv成像深度显着增加（约4倍）至1.4 mm，从而覆盖小鼠皮层的全厚度（即第I-VI层）。有趣的是，我们展示了这种独特的3D CBFv成像能力，可以识别由重复可卡因暴露引起的不同皮质层的微循环再分布。
There is growing interest in new neuroimage techniques that permit not only high-resolution quantification of cerebral blood flow velocity (CBFv) in capillaries, but also a large field of view to map the CBFv network dynamics. Such image capabilities are of great importance for decoding the functional difference across multiple cortical layers under stimuli. To tackle the limitation of optical penetration depth, we present a new ultrahigh-resolution optical coherence Doppler tomography (µODT) system at 1310 nm and compare it with a prior 800 nm µODT system for mouse brain 3D CBFv imaging. We show that the new 1310 nm µODT allows for dramatically increased depth (~4 times) of quantitative CBFv imaging to 1.4 mm, thus covering the full thickness of the mouse cortex (i.e., layers I–VI). Interestingly, we show that such a unique 3D CBFv imaging capability allows identification of microcirculatory redistribution across different cortical layers resulting from repeated cocaine exposures.