THE NUCLEOTIDE-SEQUENCE OF THE HIS4 REGION OF YEAST
THE NUCLEOTIDE-SEQUENCE OF THE HIS4 REGION OF YEAST
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DOI：
10.1016/0378-1119(82)90055-5
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发表时间：
1982-01-01
期刊：
影响因子：
3.5
通讯作者：
FINK, GR
中科院分区：
文献类型：
--
作者：
DONAHUE, TF;FARABAUGH, PJ;FINK, GR
测定了酵母HIS 4基因及其5“和3”侧翼序列的核苷酸序列。蛋白质链的计算分子量为87，935。HIS4转录物的5“末端定位在蛋白质合成起始位点上游63 bp [碱基对]的位置。HIS 4转录本的3“末端定位在位置. apprx。UAG终止密码子后118 bp。没有证据表明转录单位内存在插入序列。通过确定各种his4突变的序列变化来鉴定HIS 4编码框内的功能亚区。
The nucleotide sequence of the yeast HIS4 gene and its 5'' and 3'' flanking sequences were determined. The protein chain has a calculated MW of 87,935. The 5'' end of the HIS4 transcript maps at a position 63 bp [base pair] upstream from the site of initiation of protein synthesis. The 3'' end of the HIS4 transcript maps at a position .apprx. 118 bp after the UAG termination codon. There is no evidence for intervening sequences within the transcription unit. Functional subregions within the HIS4 coding frame were identified by determining the sequence changes for various his4 mutations.