CENP-I is essential for centromere function in vertebrate cells
CENP-I is essential for centromere function in vertebrate cells
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DOI：
10.1016/s1534-5807(02)00144-2
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发表时间：
2002-04-01
影响因子：
11.8
通讯作者：
Fukagawa, T
中科院分区：
文献类型：
--
作者：
Nishihashi, A;Haraguchi, T;Fukagawa, T
我们确定了一种新的必需的着丝粒蛋白质，CENP-I，它显示出与裂殖酵母Mis 6蛋白的序列相似性，并且我们表明CENP-I是在脊椎动物细胞的整个细胞周期中与CENP-A，-C和-H共定位的着丝粒的组成部分。为了确定CENP-I的精确功能，我们通过在鸡DT 40细胞系中产生条件性功能丧失突变体来检查其在着丝粒功能中的作用。在缺乏CENP-1的情况下，细胞在前中期因染色体错位而停滞很长一段时间。最终，细胞退出有丝分裂而不经历胞质分裂。CENP-I缺陷细胞的免疫细胞化学分析表明，CENP-I和CENP-H都是CENP-C定位所必需的，而不是CENP-A到着丝粒。
We identified a novel essential centromere protein, CENP-I, which shows sequence similarity with fission yeast Mis6 protein, and we showed that CENP-I is a constitutive component of the centromere that colocalizes with CENP-A, -C, and -H throughout the cell cycle in vertebrate cells. To determine the precise function of CENP-I, we examined its role in centromere function by generating a conditional loss-of-function mutant in the chicken DT40 cell line. In the absence of CENP-I, cells arrested at prometaphase with misaligned chromosomes for long periods of time. Eventually, cells exited mitosis without undergoing cytokinesis. Immunocytochemical analysis of CENP-I-deficient cells demonstrated that both CENP-I and CENP-H are necessary for localization of CENP-C but not CENP-A to the centromere.