Low Expression of FFAR2 in Peripheral White Blood Cells May Be a Genetic Marker for Early Diagnosis of Acute Myocardial Infarction
Low Expression of FFAR2 in Peripheral White Blood Cells May Be a Genetic Marker for Early Diagnosis of Acute Myocardial Infarction
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DOI：
10.1155/2020/3108124
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发表时间：
2020-01-25
影响因子：
2.1
通讯作者：
Meng, Fanbo
中科院分区：
文献类型：
--
作者：
Ruan, Jianjun;Meng, Heyu;Meng, Fanbo
目标。为寻找诊断急性心肌梗死的分子标志物，本研究在已有基因芯片结果的基础上，通过扩大样本量进一步验证了FFAR2基因表达水平与急性心肌梗死的关系。方法：研究方法。采集113例急性心肌梗死患者和94例非冠脉疾病患者的外周静脉血白细胞作为实验组和对照组。实时荧光定量聚合酶链式反应检测FFAR2基因的表达。Western印迹分析检测FFAR2基因在蛋白质水平的相对表达。此外，还对基因表达与临床资料的关系进行了分析和比较。结果。急性心肌梗死患者外周血中FFAR2基因的表达水平显著低于对照组(分别为0.33[0.04~1.08]，0.62[0.07~1.86]；P
Objective. To find molecular markers for the diagnosis of acute myocardial infarction (AMI), this research further verified the relationship between the expression level of FFAR2 gene and AMI by expanding the sample size based on the previous gene chip results. Methods. Peripheral venous leukocytes were collected from 113 patients with AMI and 94 patients with noncoronary artery disease as the experimental group and the control group, respectively. Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of the FFAR2 gene. Western blot analysis was applied to detect the relative expression of the FFAR2 gene at the level of protein. Furthermore, the relationship between gene expression and clinical data was also analyzed and compared. Results. The level of expression of FFAR2 gene in peripheral blood of patients with AMI was significantly lower than that of the control group (0.33 [0.04-1.08], 0.62 [0.07-1.86], respectively; p