Biodistribution of arctigenin-loaded nanoparticles designed for multimodal imaging.
Biodistribution of arctigenin-loaded nanoparticles designed for multimodal imaging.
复制标题
用于多模态成像的牛蒡苷元负载纳米颗粒的生物分布
DOI：
10.1186/s12951-017-0263-8
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发表时间：
2017-04-07
影响因子：
10.2
通讯作者：
Bai G
中科院分区：
文献类型：
--
作者：
Cui Q;Hou Y;Wang Y;Li X;Liu Y;Ma X;Wang Z;Wang W;Tao J;Wang Q;Jiang M;Chen D;Feng X;Bai G
跟踪天然产物的目标是从生药学到生物医学等领域最具挑战性的问题之一。人们普遍认为，生物相容纳米颗粒(NP)可以作为一把打开靶标“锁”的“钥匙”。我们报道了一种功能化的多赖氨酸NP技术，该技术可以在体内非侵入性地监测牛至苷元(ATG)的靶蛋白。制备了纳米粒子，并用透射电子显微镜、激光粒度分析和原子力显微镜对其形貌和表面化学性质进行了表征。此外，我们还在细胞水平和整个动物(斑马鱼和小鼠)水平上研究了ATG的定位。我们证明，荧光纳米颗粒可以作为理想的载体，用于开发一种可行的靶标识别方法。靶蛋白的分布与ATG在细胞和整个生物体水平的药理作用一致。结果表明，功能化的多赖氨酸纳米颗粒在牛至皂苷元的多模式成像中具有一定的应用价值。本文的在线版本(doi：10.1186/s12951-0170263-8)包含补充材料，授权用户可以使用。
Tracking targets of natural products is one of the most challenging issues in fields ranging from pharmacognosy to biomedicine. It is widely recognized that the biocompatible nanoparticle (NP) could function as a “key” that opens the target “lock”. We report a functionalized poly-lysine NP technique that can monitor the target protein of arctigenin (ATG) in vivo non-invasively. The NPs were synthesized, and their morphologies and surface chemical properties were characterized by transmission electron microscopy (TEM), laser particle size analysis and atomic force microscopy (AFM). In addition, we studied the localization of ATG at the level of the cell and the whole animal (zebrafish and mice). We demonstrated that fluorescent NPs could be ideal carriers in the development of a feasible method for target identification. The distributions of the target proteins were found to be consistent with the pharmacological action of ATG at the cellular and whole-organism levels. The results indicated that functionalized poly-lysine NPs could be valuable in the multimodal imaging of arctigenin. The online version of this article (doi:10.1186/s12951-017-0263-8) contains supplementary material, which is available to authorized users.