Effect of surface lipopolysaccharide on the nature of membrane vesicles liberated from the Gram-negative bacterium Pseudomonas aeruginosa
Effect of surface lipopolysaccharide on the nature of membrane vesicles liberated from the Gram-negative bacterium Pseudomonas aeruginosa
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DOI：
10.1093/jmicro/52.5.465
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发表时间：
2003-01-01
期刊：
影响因子：
--
通讯作者：
Beveridge, TJ
中科院分区：
文献类型：
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作者：
Nguyen, TT;Saxena, A;Beveridge, TJ
Membrane vesicles (MVs) were isolated from three isogenic lipopolysaccharide (LPS) mutants derived from the Gram-negative bacterium Pseudomonas aeruginosa PAO1 and compared with the parent strain. Parent cells possessed serotype 05 (A(+)B(+)) LPS whereas the three mutants contained A(+)B(-), A(-)B(+) and A(-)B(-). The MVs from the mutants contained their expected phenotypic LPS and varied in size, especially A(-)B(-) MVs. Mass and total protein differences were also noted. When the parent and mutant cells were treated with the outer membrane-perturbing antibiotic gentamicin, all cells produced 3-5 times more MVs and these had great variation in size, mass and total protein. It is concluded that the type of cellular LPS affects both the natural and gentamicin-induced development of MVs.
