Oxidative stress in testicular tissues of rats exposed to cigarette smoke and protective effects of caffeic acid phenethyl ester
Oxidative stress in testicular tissues of rats exposed to cigarette smoke and protective effects of caffeic acid phenethyl ester
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DOI：
10.1111/j.1745-7262.2006.00119.x
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发表时间：
2006-03-01
影响因子：
2.9
通讯作者：
Sarsilmaz, M
中科院分区：
文献类型：
--
作者：
Ozyurt, H;Pekmez, H;Sarsilmaz, M
目的：观察香烟烟雾暴露对大鼠睾丸组织的氧化应激作用，并探讨咖啡酸苯乙酯（CAPE）的干预作用。方法：21只大鼠分为3组，每组7只。第I组动物用作对照。组II中的大鼠仅暴露于香烟烟雾（4 x 30 min/d），组III中的大鼠暴露于香烟烟雾并接受每日腹膜内注射CAPE（10 μ mol/kg-d）。60 d后处死大鼠，用分光光度法测定睾丸组织中一氧化氮（NO）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）等抗氧化酶及丙二醛（MDA）含量。结果如下：有一个显着的增加，过氧化氢酶和超氧化物歧化酶的活性在组II相比，对照组，但两者的水平下降后，CAPE管理组III。GSH-Px活性在组II中降低，但CAPE引起GSH-Px活性在组III中升高。GSH-Px在Ⅰ组与Ⅱ组间差异有显著性，Ⅱ组与Ⅲ组间差异无显著性。烟雾暴露后丙二醛的升高是显着的，CAPE引起的水平下降，这是没有统计学差异的对照组。一个显着增加的NO水平后，暴露于烟雾被逆转CAPE管理和NO水平之间的差异，在组I和III是统计学上不显着的。结论：香烟烟雾暴露可引起大鼠睾丸组织中氧化酶水平的变化，而CAPE可逆转这些有害作用。
Aim: To show the oxidative stress after cigarette smoke exposure in rat testis and to evaluate the effects of caffeic acid phenethyl ester (CAPE). Methods: Twenty-one rats were divided into three groups of seven. Animals in Group I were used as control. Rats in Group II were exposed to cigarette smoke only (4 x 30 min/d) and rats in Group III were exposed to cigarette smoke and received daily intraperitoneal injections of CAPE (10 mu mol/kg-d). After 60 days all the rats were killed and the levels of nitric oxide (NO) and anti-oxidant enzymes such as superoxide-dismutase, catalase and glutathione peroxidase (GSH-Px) and the level of malondialdehyde were studied in the testicular tissues of rats with spectrophotometric analysis. Results: There was a significant increase in catalase and superoxide-dismutase activities in Group II when compared to the controls, but the levels of both decreased after CAPE administration in Group III. GSH-Px activity was decreased in Group II but CAPE caused an elevation in GSH-Px activity in Group III. The difference between the levels of GSH-Px in Group I and Group II was significant, but the difference between groups II and III was not significant. Elevation of malondialdehyde after smoke exposure was significant and CAPE caused a decrease to a level which was not statistically different to the control group. A significantly increased level of NO after exposure to smoke was reversed by CAPE administration and the difference between NO levels in groups I and III was statistically insignificant. Conclusion: Exposure to cigarette smoke causes changes in the oxidative enzyme levels in rat testis, but CAPE can reverse these harmful effects.