Nanoscale topography modulates corneal epithelial cell migration
Nanoscale topography modulates corneal epithelial cell migration
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DOI：
10.1002/jbm.a.30467
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发表时间：
2005-12-01
影响因子：
4.9
通讯作者：
Murphy, CJ
中科院分区：
文献类型：
--
作者：
Diehl, KA;Foley, JD;Murphy, CJ
本研究的目的是评估模拟角膜上皮基底膜的表面地形特征对细胞迁移的影响。我们使用电子束和X射线光刻以及反应离子蚀刻技术，在硅片上制作出具有纳米和微米级尺寸间距（凹槽宽度加脊宽度，间距范围从400到4000纳米）的图案。此外，通过复制模塑技术制作了聚氨酯图案化表面，以便对迁移细胞进行实时成像。单个SV40转化的人角膜上皮细胞经常与下方的表面图案对齐，并且几乎完全沿着所有间距的凹槽和脊迁移。单个细胞在光滑表面上的迁移方向是随机的。在细胞分散试验中，细胞集落主要沿着凹槽和脊从最初的圆形区域迁移出来，尽管也有一些垂直于脊的迁移。在光滑表面上，细胞呈放射状向各个方向均匀迁移，保持圆形集落形状。我们得出结论，类似于天然基底膜的基质特征可调节角膜上皮细胞的迁移。这些发现与角膜内稳态的维持和伤口愈合以及组织工程、角膜假体开发和细胞培养材料制造策略的演变有关。（c）2005威利期刊公司
The purpose of this study was to evaluate the effect of surface topographic features that mimic the corneal epithelial basement membrane on cell migration. We used electron-beam and X-ray lithography and reactive ion etching to pattern silicon wafers with pitches (groove width Plus ridge width) of nano- and microscale dimensions (pitches ranged from 400 to 4000 nm). Additionally, polyurethane patterned surfaces were created by replication molding techniques to allow for real-time imaging of migrating cells. Individual SV40-transformed human corneal epithelial cells frequently aligned with respect to the underlying surface patterns and migrated almost exclusively along grooves and ridges of all pitches. Direction of migration of individual cells on smooth surfaces was random. In cell dispersion assays, colonies of cells migrated out from initially circular zones predominantly along grooves and ridges, although there was some migration perpendicular to the ridges. On smooth surfaces, cells migrated radially, equally in all directions, maintaining circular colony shapes. We conclude that Substratum features resembling the native basement membrane modulate corneal epithelial cell migration. These findings have relevance to the maintenance of corneal homeostasis and wound healing, as well as to the evolution of strategies in tissue engineering, corneal prosthesis development, and cell culture material fabrication. (c) 2005 Wiley Periodicals, Inc.