Comparison of adrenoceptor subtype expression in porcine and human bladder and prostate
Comparison of adrenoceptor subtype expression in porcine and human bladder and prostate
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DOI：
10.1007/bf00941983
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发表时间：
1997-06-01
影响因子：
--
通讯作者：
Michel, MC
中科院分区：
文献类型：
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作者：
Goepel, M;Wittmann, A;Michel, MC
我们对猪膀胱逼尿肌和膀胱颈、人膀胱逼尿肌以及猪和人前列腺中的α(1)-、α(2)-和β-肾上腺素受体亚型进行了量化和表征。分别使用[H-3]哌唑嗪、[H-3]RX 821002和[I-125]io多氰吲哚洛尔作为放射性配体，在放射性配体结合研究中鉴定了α(1)-、α(2)-和β-肾上腺素受体。在猪雄性和雌性逼尿肌、膀胱颈和雄性前列腺中，检测到的肾上腺素受体丰度顺序为β > α(2)，远大于α(1)（未检测到），性别之间或逼尿肌和膀胱颈之间没有重大差异。在人类逼尿肌和前列腺中，丰度顺序分别是β>α(2)远大于α(1)(检测不到)，β远大于α(1)>α(2)。所有组织中的 α(2)-肾上腺素受体均属于 α(2A) 亚型，如育亨宾、哌唑嗪、ARC 239 和羟甲唑啉的竞争结合研究所证明。 β-肾上腺素受体代表在所有组织中以 β(2) 亚型为主的混合群体，如与 ICI 118,551 和 CGP 20,712A 的竞争结合所证明的。我们的结论是，猪可能是研究逼尿肌功能与肾上腺素受体表达相关的合适模型。它们可能不太适合膀胱颈或前列腺功能的研究。
We have quantified and characterized alpha(1)-, alpha(2)- and beta-adrenoceptor subtypes in porcine bladder detrusor and bladder neck, human bladder detrusor, and porcine and human prostate. alpha(1)-, alpha(2)- and beta-adrenoceptor were identified in radioligand binding studies using [H-3]prazosin, [H-3]RX 821002 and [I-125]io docyanopindolol, respectively, as the radioligands. In porcine male and female detrusor and bladder neck and male prostate, adrenoceptors were detected in the order of abundance beta > alpha(2) much greater than alpha(1) (not detectable), with no major differences between the sexes or between detrusor and bladder neck. In human detrusor and prostate the order of abundance was beta > alpha(2) much greater than alpha(1) (not detectable) and beta much greater than alpha(1) > alpha(2), respectively. The alpha(2)-adrenoceptors in all tissues were homogeneously of the alpha(2A)-subtype as evidenced by competition binding studies with yohimbine, prazosin, ARC 239 and oxymetazoline. The beta-adrenoceptors represented a mixed population with a dominance of the beta(2)-subtype in all tissues as demonstrated by competition binding with ICI 118,551 and CGP 20,712A. We conclude that pigs may be a suitable model for studies of detrusor function with respect to adrenoceptor expression. They may be less suitable for studies of bladder neck or prostate function.