Detection of hypophosphite, phosphite, and orthophosphate in natural geothermal water by ion chromatography
Detection of hypophosphite, phosphite, and orthophosphate in natural geothermal water by ion chromatography
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DOI：
10.1016/j.chroma.2003.11.056
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发表时间：
2004-06-11
影响因子：
4.1
通讯作者：
Foster, KL
中科院分区：
文献类型：
--
作者：
McDowell, MM;Ivey, MM;Foster, KL
目前的理论指出，磷以五价（V）氧化态作为正磷酸盐掺入细胞中。然而，最近的研究表明，微生物含有用于代谢还原形式的磷的酶，包括亚磷酸盐（III）和次磷酸盐（I），这表明这些化学物质存在天然来源。本文将讨论抑制电导离子色谱法检测次磷酸盐，亚磷酸盐，正磷酸盐在地热水基质含有氟，氯，溴，硝酸盐，碳酸氢盐和硫酸盐。所有的峰被清楚地解决，校准是线性的，估计3sigma检测限为0.83，0.39，和0.35 μ M的次磷酸盐，亚磷酸盐，和正磷酸盐，分别。(C)2003 Elsevier B.V.保留所有权利。
Current doctrine states that phosphorus is incorporated into cells in the pentavalent(V) oxidation state as orthophosphate. However, recent studies show that microorganisms contain enzymes used to metabolize reduced forms of phosphorous, including phosphite(III) and hypophosphite(I), which suggests that there is a natural source for these chemical species. This paper will discuss suppressed conductivity ion chromatography methods developed to detect hypophosphite, phosphite, and orthophosphate in a geothermal water matrix containing fluoride, chloride, bromide, nitrate, hydrogen carbonate and sulfate. All peaks were clearly resolved, and calibrations were linear with estimated 3sigma detection limits of 0.83, 0.39, and 0.35 muM for hypophosphite, phosphite, and orthophosphate, respectively. (C) 2003 Elsevier B.V. All rights reserved.