DEVELOPMENT OF BOVINE EMBRYOS IN-VITRO AS AFFECTED BY ENERGY SUBSTRATES
DEVELOPMENT OF BOVINE EMBRYOS IN-VITRO AS AFFECTED BY ENERGY SUBSTRATES
复制标题
DOI：
10.1095/biolreprod49.3.459
复制
发表时间：
1993-09-01
影响因子：
3.6
通讯作者：
FIRST, NL
中科院分区：
文献类型：
--
作者：
ROSENKRANS, CF;ZENG, GQ;FIRST, NL
开发了简单的培养基来研究体外牛胚胎直至第 7 天（第 0 天 = 卵母细胞抽吸日）的代谢需求。胚胎来源于在体外成熟并受精的卵母细胞。授精后 45 +/- 2 小时，胚胎（大于或等于 2 个细胞）被随机分配至治疗组。实验1和实验3检查了在乳酸存在下丙酮酸浓度的影响。在存在乳酸的情况下，丙酮酸 (0.2-5.0 mM) 对桑葚或胚泡的百分比没有影响 (p > 0.05)。然而，将 L-乳酸半钙的浓度从 5 mM 增加到 10 mM 会降低 (p < 0.001) 达到桑葚胚或囊胚阶段的胚胎百分比（实验 3）。当添加到培养基 CR1 中时，硫酸镁 (0.5 mM) 和 EDTA (10 mM) 都不会改善胚胎发育（实验 2）。将钙水平增加至 5 mM 或将乳酸水平增加至 10 mM 对胚胎发育没有影响 (p > 0.05)（实验 4）。然而，添加钙和乳酸的相互作用导致桑葚籽百分比下降（p < 0.05）。实验6确定了丙酮酸、乳酸和葡萄糖对体外胚胎发育的独立影响。随着丙酮酸或乳酸水平从 1 mM 增加至 10 mM，囊胚百分比下降 (p < 0.05)。这些实验表明，在含有乳酸盐的培养基中添加丙酮酸盐对于牛胚胎的体外发育不是必需的。
Simple media were developed to study the metabolic requirements of bovine embryos up to Day 7 (Day 0 = day of oocyte aspiration) in vitro. Embryos were derived from oocytes matured and fertilized in vitro. At 45 +/- 2 h post insemination, embryos (greater-than-or-equal-to 2 cells) were randomly allotted to treatments. Examined in experiments 1 and 3 was the effect of pyruvate concentration in the presence of lactate. In the presence of lactate, pyruvate (0.2-5.0 mM) had no effect (p > 0.05) on the percentage of morulae or blastocysts. However, increasing the concentration of hemicalcium L-lactate from 5 mM to 10 mM decreased (p < 0.001) the percentage of embryos reaching the morula or blastocyst stage (experiment 3). Neither magnesium sulfate (0.5 mM) nor EDTA (10 mM) improved embryo development when added to the medium CR1 (experiment 2). Increasing the calcium level to 5 mM or the lactate level to 10 mM had no effect (p > 0.05) on embryo development (experiment 4). However, the interaction of adding calcium and lactate resulted in a decreased (p < 0.05) percentage of morulae. Determined in experiment 6 were the independent effects of pyruvate, lactate, and glucose on embryo development in vitro. As pyruvate or lactate level was increased from 1 to 10 mM, the percentage of blastocysts was decreased (p < 0.05). These experiments indicate that adding pyruvate to a medium containing lactate is not necessary for development of bovine embryos in vitro.