A Universal Boronate-Affinity Crosslinking-Amplified Dynamic Light Scattering Immunoassay for Point-of-Care Glycoprotein Detection
A Universal Boronate-Affinity Crosslinking-Amplified Dynamic Light Scattering Immunoassay for Point-of-Care Glycoprotein Detection
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DOI：
10.1002/anie.202112031
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发表时间：
2021-12-23
影响因子：
16.6
通讯作者：
Tang, Ben Zhong
中科院分区：
文献类型：
--
作者：
Chen, Jing;Hao, Liangwen;Tang, Ben Zhong
在此，我们报告了一种通用的硼酸盐亲和交联放大动态光散射（DLS）免疫分析法，用于复杂样品的即时（POC）糖蛋白检测。这种增强的DLS免疫分析由两个元素组成，即抗体包被的磁性纳米颗粒（MNP@mAb）用于目标捕获和DLS信号转导，以及基于苯硼酸的硼酸亲和材料作为交联放大器。添加靶标后，糖蛋白首先通过MNP@mAb捕获，并通过靶诱导交联扩增，这是由硼酸配体与含顺式二醇的糖蛋白之间的选择性结合引起的，从而导致平均纳米颗粒大小的DLS信号显着增加。利用糖蛋白与交联剂之间的多价结合和快速硼酸盐亲和反应，该免疫传感策略实现了15 min内fM水平糖蛋白的超灵敏快速定量检测。总的来说，这项工作为扩展DLS技术提供了一个有前途的和通用的设计策略，即使在现场或POC也可以检测糖蛋白。
Herein, we report a universal boronate-affinity crosslinking-amplified dynamic light scattering (DLS) immunoassay for point-of-care (POC) glycoprotein detection in complex samples. This enhanced DLS immunoassay consists of two elements, i.e., antibody-coated magnetic nanoparticles (MNP@mAb) for target capture and DLS signal transduction, and phenylboronic acid-based boronate-affinity materials as crosslinking amplifiers. Upon the addition of targets, glycoproteins are first captured by MNP@mAb and amplified by target-induced crosslinking stemming from the selective binding between the boronic acid ligand and cis-diol-containing glycoprotein, thereby resulting in a remarkably increased DLS signal in the average nanoparticle size. Benefiting from the multivalent binding and fast boronate-affinity reaction between glycoproteins and crosslinkers, the proposed immunosensing strategy has achieved the ultrasensitive and rapid quantitative assay of glycoproteins at the fM level within 15 min. Overall, this work provides a promising and versatile design strategy for extending the DLS technique to detect glycoproteins even in the field or at POC.