Virulence properties of motile aeromonads isolated from farmed frogs Rana tigerina and R. rugulosa
Virulence properties of motile aeromonads isolated from farmed frogs Rana tigerina and R. rugulosa
复制标题
DOI：
10.3354/dao040185
复制
发表时间：
2000-04-20
影响因子：
1.4
通讯作者：
Inglis, V
中科院分区：
文献类型：
--
作者：
Pearson, MD;Hirono, I;Inglis, V
比较了从临床正常和败血症养殖青蛙从泰国分离的气单胞菌属的毒力因子。不同气单胞菌对蛙红细胞的溶血活性存在显著差异。高溶血活性组（未种属气单胞菌，Au）、中等溶血活性组（A，A.A.）和低溶血活性组（A. veronii biovar sobria，A. veronii biovar veronii，A.豚鼠A. Schubertii）。DNA菌落杂交研究显示，Au分离株具有溶血素基因（ASH1），该基因在任何其他泰国气单胞菌或供试典型菌株中均不存在。90%的Au分离株、60%的A.在其他活动的气单胞菌中没有发现。气单胞菌分离株的细胞毒性活性根据测定中使用的细胞来源而变化。虹鳟鱼的细胞对Au毒素非常敏感，但对其他物种产生的毒素不太敏感。相反，哺乳动物细胞对Au毒素的敏感性很低，但对A.你好因此，选择合适的测定底物是重要的。
Virulence factors were compared in Aeromonas species isolated from clinically normal and septicaemic farmed frogs from Thailand. Haemolysin activities against frog erythrocytes were significantly different within the collection of aeromonads. Groups of high haemolytic activity (unspeciated Aeromonas, Au), moderate haemolytic activity (A, hydrophila), and low haemolytic activity (A. veronii biovar sobria, A. veronii biovar veronii, A. caviae, A. schubertii) were noted. DNA colony hybridisation studies revealed that Au isolates possessed a haemolysin gene (ASH1) which was not present in any of the other Thai aeromonads or type strains tested. Elastinolytic activity was demonstrated in 90% of the Au isolates, 60% of the A. hydrophila isolates and in none of the other motile aeromonads. The cytotoxic activity of the Aeromonas isolates varied according to the source of cells used in the assays. Cells from rainbow trout were extremely sensitive to Au toxins but less so to toxins produced by other species. In contrast mammalian cells showed very little sensitivity to Au toxins but were more sensitive to toxins produced by A. hydrophila. Selection of suitable assay substrates is therefore important.