Polyamine analysis of methanogens, thermophiles and extreme halophiles belonging to the domain Archaea
Polyamine analysis of methanogens, thermophiles and extreme halophiles belonging to the domain Archaea
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属于古细菌领域的产甲烷菌、嗜热菌和极端嗜盐菌的多胺分析
DOI：
10.3118/jjse.6.25
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
T. Itoh
中科院分区：
文献类型：
--
作者：
K. Hamana;Ryuichi Hosoya;T. Itoh
采用高效液相色谱法对61株新发现的古细菌细胞多胺进行了分析，以评价多胺谱与其极端微生物和化学分类的相关性。在Methanocaldococcus和Methanotorris属于Methanocaldococcaceae，但在Methanococcaceae和任何其他产甲烷古菌目中均未发现季支五胺N4-bis（氨丙基）亚精胺。热球菌目的超嗜热/极嗜热古球菌、热球菌和焦球菌普遍含有支链五胺。中温、极端嗜盐菌，包括嗜碱菌，属于盐杆菌目，含有微量亚精胺、精胺和精氨酸。在绿原门中，临时目“Caldisphaerales”的极端嗜热的Caldisphaera含有去亚精胺、亚精胺和胍丁胺，而属于热蛋白酶目的超嗜热的Pyrobaculum含有去亚精胺、亚精胺、去精胺和精胺，但不含任何五胺。在Desulfurococcales目中，Desulfurococcus和Ignisphaera含有亚精胺、去亚精胺、同亚精胺、去精胺和精胺，而Aeropyrum除了这些三胺和四胺外还含有钙多巴胺和热敏多巴胺。
Cellular polyamines of 61 archaeal newly published strains were analyzed by HPLC to evaluate correlation of polyamine profiles to their extremophily and chemotaxonomy. A quaternary branched penta-amine, N4-bis( aminopropyl )spermidine, was found in hyperthermophilic Methanocaldococcus and Methanotorris belonging to the family Methanocaldococcaceae but not in the family Methanococcaceae nor any other methanogenic archaeal orders. Hyperthermophilic / extremely thermophilic Palaeococcus, Thermococcus and Pyrococcus of the order Thermococcales ubiquitously contained the branched penta-amine. Mesophilic, extreme halophiles including halo-alkaliphiles, belonging to the order Halobacteriales, contained a trace of spermidine, spermine and agmatine. In the phylum Crenarchaeota, extremely thermophilic Caldisphaera of the provisional order 'Caldisphaerales' contained norspermidine, spermidine and agmatine, and hyperthermophilic Pyrobaculum belonging to the order Thermoproteales contained norspermidine, spermidine, norspermine and spermine, but not any penta-amines. Within the order Desulfurococcales, Desulfurococcus and Ignisphaera contained spermidine, norspermidine, homospermidine, norspermine and spermine, whereas Aeropyrum contained caldopentamine and thermopentamine in addition to these triamines and tetra-amines.