Mineralization of ytterbium-doped hydroxyapatite nanorod arrays in magnetic chitosan scaffolds improves osteogenic and angiogenic abilities for bone defect healing
Mineralization of ytterbium-doped hydroxyapatite nanorod arrays in magnetic chitosan scaffolds improves osteogenic and angiogenic abilities for bone defect healing
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磁性壳聚糖支架中掺镱羟基磷灰石纳米棒阵列的矿化可改善骨缺损愈合的成骨和血管生成能力
DOI：
10.1016/j.cej.2020.124166
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发表时间：
2020-05
影响因子：
15.1
通讯作者：
Guo Ya-Ping
中科院分区：
文献类型：
--
作者：
Tang Ya-Qi;Wang Qi-Yang;Ke Qin-Fei;Zhang Chang-Qing;Guan Jun-Jie;Guo Ya-Ping
羟基磷灰石（hydroxyapatite，HA）是一种传统的骨修复材料，但其成骨和血管生成能力差，阻碍了其在临床上应用于骨缺损的修复。在此，将掺镱羟基磷灰石（YbHA）纳米棒阵列原位沉积在磁性壳聚糖（CS）多孔支架中，从而产生磁性YbHA/CS（MYbHA/CS）纳米杂化支架。我们假设磁性SrFe_（12）O_（19）纳米片和Yb掺杂的功能支架有利于骨缺损区的成骨和血管化。为了验证这一假设，以大鼠骨髓间充质干细胞（rBMSCs）和大鼠颅骨缺损为模型，研究MYbHA/CS支架的成骨和血管生成活性。幸运的是，来自MYbHA/CS支架的磁场和Yb 3+离子都通过激活BMP-2/Smad通路显著上调成骨相关基因的表达，在活体颅骨缺损模型中进一步显示，磁性SrFe 12 O 19纳米片和Yb掺杂剂的联合作用显著刺激了血管内皮生长因子的表达。血管和新骨的生长进入纳米混合支架中的三维通道。因此，将SrFe_（12）O_（19）纳米片和Yb掺杂物修饰于骨支架材料中，是一种促进骨缺损愈合的新方法。
Hydroxyapatite (HA) is a traditional bone repair material, but its poor osteogenic and angiogenic abilities hinder the clinical application for critical-sized bone defect healing. Herein, ytterbium-doped hydroxyapatite (YbHA) nanorod arraysin situdeposited in magnetic chitosan (CS) porous scaffolds, leading to create magnetic YbHA/CS (MYbHA/CS) nanohybrid scaffolds. We hypothesized that both magnetic SrFe12O19nanoplates and Yb dopants in the functional scaffolds favored the osteogenesis and vascularization in bone defect regions. To prove the hypothesis, the osteogenic and angiogenic activities of MYbHA/CS scaffolds were investigated by using rat bone marrow mesenchymal stem cells (rBMSCs) and rat cranial defects as models. Fortunately, both the magnetic fields and Yb3+ions as-derived from MYbHA/CS scaffolds remarkably up-regulated the expression of osteogenic-related genes by activating BMP-2/Smad pathway, and simultaneously increased the expression of vascular endothelial growth factor.In vivorat cranial defect models further revealed that the combination effects between magnetic SrFe12O19nanoplates and Yb dopants remarkably stimulated the in-growth of blood vessels and new bones into three-dimensional channels in the nanohybrid scaffolds. Hence, the modification of SrFe12O19nanoplates and Yb dopants in bone scaffolds is a novel and promising strategy to accelerate bone defect healing.
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影响因子：
14
作者：
Sui L;Wang M;Han Q;Yu L;Zhang L;Zheng L;Lian J;Zhang J;Valverde P;Xu Q;Tu Q;Chen J
通讯作者：
Chen J
影响因子：
4.7
作者：
Jiawei Lu;Yu Miao;Cui‐Xiang Guo;Qinfei Ke;J. Yin;Shumin Zhou;Yaping Guo
通讯作者：
Jiawei Lu;Yu Miao;Cui‐Xiang Guo;Qinfei Ke;J. Yin;Shumin Zhou;Yaping Guo
影响因子：
14
作者：
Spiller, Kara L.;Anfang, Rachel R.;Spiller, Krista J.;Ng, Johnathan;Nakazawa, Kenneth R.;Daulton, Jeffrey W.;Vunjak-Noyakovic, Gordana
通讯作者：
Vunjak-Noyakovic, Gordana
影响因子：
9.4
作者：
Luz A. Zavala;P. Fernández;E. Novitskaya;Jorge N. Díaz;M. Herrera;O. Graeve
通讯作者：
Luz A. Zavala;P. Fernández;E. Novitskaya;Jorge N. Díaz;M. Herrera;O. Graeve
影响因子：
2.5
作者：
Araceli Mauricio-Sanchez, Reina;Salazar, Ricardo;Mendoza-Galvan, Arturo
通讯作者：
Mendoza-Galvan, Arturo