RNA OF MOUSE HEPATITIS VIRUS
RNA OF MOUSE HEPATITIS VIRUS
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DOI：
10.1128/jvi.26.2.236-242.1978
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发表时间：
1978-01-01
影响因子：
5.4
通讯作者：
STOHLMAN, SA
中科院分区：
文献类型：
--
作者：
LAI, MMC;STOHLMAN, SA
小鼠肝炎病毒是一种冠状病毒，从感染早期释放的病毒中分离RNA，并通过蔗糖梯度沉降和电泳进行分析。它由一段约60 S的单链RNA组成。其MW为5.4 × 104。106通过在甲基汞-琼脂糖凝胶中电泳。至少1/3的RNA含有多聚腺苷酸化序列。因此，它可能是积极的搁浅。在感染后期收获的病毒除了含有60 S外，还含有一些30- 50 S RNA，它们可能是60 S RNA的降解产物。从致病性（JHM）和非致病性（A59）菌株分离的RNA之间的电泳行为没有差异。
The RNA of mouse hepatitis virus, a coronavirus, was isolated from the virus released early in the infection and analyzed by sucrose gradient sedimentation and electrophoresis. It consisted of a piece of single-stranded RNA of about 60S. Its MW was 5.4 .times. 106 by electrophoresis in methylmercury-agarose gels. At least 1/3 of the RNA contained polyadenylated sequences. It thus probably positive stranded. The virus harvested late in the infection contained, bssides 60S, some 30-50S RNA that are possibly degradation products of the 60S RNA. No difference in the electrophoretic behavior could be detected between the RNA isolated from a pathogenic (JHM) and a nonpathogenic (A59) strain.