Microcystin-LR affects the hypothalamic-pituitary-inter-renal (HPI) axis in early life stages (embryos and larvae) of zebrafish
Microcystin-LR affects the hypothalamic-pituitary-inter-renal (HPI) axis in early life stages (embryos and larvae) of zebrafish
复制标题
微囊藻毒素-LR 影响斑马鱼早期生命阶段（胚胎和幼虫）的下丘脑-垂体-肾间 (HPI) 轴
DOI：
10.1016/j.envpol.2018.05.024
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发表时间：
2018-10-01
影响因子：
8.9
通讯作者：
Xie, Ping
中科院分区：
文献类型：
--
作者：
Chen, Liang;Wang, Yeke;Xie, Ping
蓝藻水华的频率和持续时间都在增加。一些蓝藻可以产生包括微囊藻毒素（MCs）在内的蓝藻毒素。微囊藻是有害藻华最常见的有毒产物，具有最大的暴露和毒性潜力。最近，MC已被证明会扰乱内分泌功能。在这项研究中，第一次，MC-LR的影响下丘脑-垂体-肾间（HPI）轴在早期胚胎发育（胚胎/幼虫）的斑马鱼（Danio rerio），进行了调查。在受精后4-168 h（hpf）内，将斑马鱼胚胎/幼体暴露于1、10、100和300 ~（14）MC-LR/L。暴露于300 μ g MC-LR/L导致全身皮质醇浓度显著高于对照组。沿着HPI轴和盐皮质激素受体（MR-）和糖皮质激素受体（GR-）为中心的基因网络的基因表达进行了评估，通过使用定量实时PCR的crh的mRNA表达显着下调暴露于300 μ g MC-LR/L，而crhbp，crhr 1，crhr 2的表达显着上调，相对于对照组。MC-LR导致类固醇生成基因包括hmgra、星星和cyp 17的mRNA水平显著降低，但hsd 20 b的mRNA表达显著高于对照组。MC-LR治疗也改变了MR和GR为中心的基因网络的转录谱，这可能导致多种反应。综上所述，这些结果表明，MC-LR影响斑马鱼幼虫的皮质类固醇-内分泌系统。这项研究为了解MCs潜在毒性和内分泌干扰的分子机制提供了有价值的见解。(C)2018爱思唯尔有限公司版权所有
Frequencies and durations of blooms of cyanobacteria are increasing. Some cyanobacteria can produce cyanotoxins including microcystins (MCs). MCs are the most common toxic products of hazardous algal blooms (HABs), with the greatest potential for exposure and to cause toxicity. Recently, MCs have been shown to disrupt endocrine functions. In this study, for the first time, effects of MC-LR on the hypothalamic-pituitary-inter-renal (HPI) axis during early embryonic development (embryos/larvae) of zebrafish (Danio rerio), were investigated. Embryos/larvae of zebrafish were exposed to 1, 10, 100, or 30014 MC-LR/L during the period of 4-168 h post-fertilization (hpf). Exposure to 300 mu g MC-LR/L resulted in significantly greater concentrations of whole-body cortisol than those in controls. Expressions of genes along the HPI axis and mineralocorticoid receptor (MR-) and glucocorticoid receptor (GR-) centered gene networks were evaluated by use of quantitative real-time PCR Expression of mRNA for crh was significantly down-regulated by exposure to 300 mu g MC-LR/L, while expressions of crhbp, crhr1, and crhr2 were significantly up-regulated, relative to controls. MC-LR caused significantly lesser levels of mRNA for steroidogenic genes including hmgra, star, and cyp17, but expression of mRNA for hsd20b was significantly greater than that of controls. Treatment with MC-LR also altered profiles of transcription of MR- and GR-centered gene networks, which might result in multiple responses. Taken together, these results demonstrated that MC-LR affected the corticosteroid-endocrine system of larvae of zebrafish. This study provided valuable insights into molecular mechanisms behind potential toxicity and endocrine disruption of MCs. (C) 2018 Elsevier Ltd. All rights reserved.