Nanoparticles affect the survival of bacteria on leaf surfaces.
Nanoparticles affect the survival of bacteria on leaf surfaces.
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DOI：
10.1111/j.1574-6941.2009.00664.x
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发表时间：
2009-05
影响因子：
4.2
通讯作者：
Shuang Wang;Lifung Chang;Yongjun Wang;Qi Wang;Ching-Hong Yang;Ruhong Mei
中科院分区：
文献类型：
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作者：
Shuang Wang;Lifung Chang;Yongjun Wang;Qi Wang;Ching-Hong Yang;Ruhong Mei
随着纳米材料和纳米产品的日益增多，有必要更多地了解这些材料对人类健康和环境的相关影响。在本研究中，研究了纳米材料TiO2对层状球微生物群落的影响。pcr -变性梯度凝胶电泳分析结果显示tio2诱导微生物群落结构发生变化。以蜡样芽孢杆菌905为研究对象，研究了超氧化物歧化酶编码基因sodA-1和sodA-2的作用及其在TiO2光催化下的存活情况。结果表明，光催化氧化诱导了sodA-1和sodA-2的表达，其中sodA-2的诱导作用更强。此外，与野生型蜡样芽孢杆菌905相比，在喷洒TiO2的黄瓜叶片表面上，发现碱-1和碱-2双缺失突变株KOS的细菌数量减少。由于层状圈被认为是微生物的主要栖息地之一，并且地球的大部分地区都被叶子覆盖，因此本工作的结果提供了光催化纳米材料在环境中的潜在影响的信息。
The increasing presence of nanomaterial and nanoproducts makes it imperative to learn more about the associated impacts of these materials on human health and the environment. In this study, the effect of the nanomaterial TiO2 on the phyllosphere microbial community was investigated. Analysis results by PCR-denaturing gradient gel electrophoresis revealed a TiO2-induced change in the community structure of microorganisms. An epiphytic bacterium, Bacillus cereus 905, was chosen to study the role of the superoxide dismutase-encoding genes, sodA-1 and sodA-2, and its survival from TiO2 photocatalysis. Our results showed that the expression of sodA-1 and sodA-2 was induced by photocatalytic oxidation, with a higher induction observed in sodA-2. In addition, compared with wild-type B. cereus 905, a reduced bacterial population was observed in a sodA-1 and sodA-2 double deletion mutant strain KOS on a cucumber leaf surface sprayed with TiO2. Because the phyllosphere is considered as one of the major habitats for microorganisms, and substantial areas of the earth are covered with leaves, the results of this work provides information of the potential impact of photocatalytic nanomaterial in the environment.