Identification and characterization of functional subunits of Clostridium botulinum type A progenitor toxin involved in binding to intestinal microvilli and erythrocytes
Identification and characterization of functional subunits of Clostridium botulinum type A progenitor toxin involved in binding to intestinal microvilli and erythrocytes
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DOI：
10.1016/s0014-5793(00)01147-9
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发表时间：
2000-02-11
期刊：
影响因子：
3.5
通讯作者：
Oguma, K
中科院分区：
文献类型：
--
作者：
Fujinaga, Y;Inoue, K;Oguma, K
A型肉毒梭菌血凝素阳性祖毒素由三种不同的组分组成：神经毒素（NTX）、血凝素（HA）和无毒非HA（NTNH）。HA由指定为HA 1、2、3a和3b的四个子组件组成。HA阳性前体毒素GST-HA_1和GST-HA_3b与人红细胞和豚鼠小肠微绒毛结合，HA阳性前体毒素GST-HA_1通过半乳糖结合，GST-HA_3b通过唾液酸结合，GST-HAZ和GST-HA_3a未检测到结合。(C)2000年欧洲生物化学学会联合会。
Clostridium botulinum type A hemagglutinin-positive progenitor toxin consists of three distinct components: neurotoxin (NTX), hemagglutinin (HA), and non-toxic non- HA (NTNH). The HA consists of four subcomponents designated HA1, 2, 3a and 3b. By employing purified toxin and GST-fusion proteins of each HA subcomponent, we found that the HA-positive progenitor toxin, GST-HA1 and GST-HA3b bind to human erythrocytes and microvilli of guinea pig upper small intestinal sections, The HA-positive progenitor toxin and GST-HA1 bind via galactose moieties, GST-HA3b binds via sialic acid moieties, GST-HAZ and GST-HA3a showed no detectable binding. (C) 2000 Federation of European Biochemical Societies.