Visual Scorings of Drought Stress Intensity as Covariates for Improved Variety Trial Analysis

Visual Scorings of Drought Stress Intensity as Covariates for Improved Variety Trial Analysis
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干旱胁迫强度的视觉评分作为改进品种试验分析的协变量

DOI:
10.1111/jac.12025
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发表时间:
2013
影响因子:
3.5
通讯作者:
Piepho
Piepho
中科院分区:
农林科学2区
文献类型:
--
作者:
Mühleisen;Maurer;Möhring;Piepho

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非生物胁迫可增强田间的异质性,导致品种试验中对基因型效应的估计不精确。本研究是在小黑麦多点田间试验的基础上进行的。六个地点中的两个受到干旱胁迫的影响,并表现出增加的田间异质性。在Willstätt,干旱胁迫强度进行了两次视觉评分,在伊苏敦,一个情节的整体印象进行了一次评分。我们研究了视觉评分是否可以用作协方差分析(ancova)中的协变量,以消除不同干旱胁迫强度对小区产量的影响。为了评价协变量模型,我们检验了协变量是否独立于基因型效应,并与籽粒产量线性相关。此外,ancovamodels进行了比较,基线和空间模型的基础上AIC和表型相关性的基因型平均值估计的模型在干旱胁迫的位置与基因型平均值计算在其余的位置。我们发现,Willstätt的两个评分都符合anancova的要求,并导致广义遗传力(h2)和效率的增加。第二个评分的anancova将h2从基线模型的0.03增加到0.60,而最佳空间模型的h2仅增加到0.50。在伊苏敦的评分不是独立的基因型效应和减少表型相关性。我们的结论是,环境因素造成空间上不同的产量潜力可以得分或测量,并作为协变量,以获得更精确的基因型估计。
Abiotic stress can enhance the heterogeneity of fields, which leads to imprecise estimates of genotypic effects in variety trials. Our study is based on multilocation field trials of triticale (×TriticosecaleWittm.). Two of the six locations were affected by drought stress and showed increased field heterogeneity. At Willstätt, drought stress intensity was visually scored twice, and at Issoudun, overall impression of a plot was scored once. We investigated if the visual scorings can be used as covariates in an analysis of covariance (ancova) to eliminate the influence of different drought stress intensity on plot yields. For evaluation of theancovamodels, we examined if the covariates were independent from the genotypic effects and linearly associated with grain yield. In addition,ancovamodels were compared with baseline and spatial models based on AIC and phenotypic correlation between genotype means estimated with the model under investigation in a drought stress location with genotype means calculated across the remaining locations. We found that both scorings in Willstätt fulfilled the requirements of anancovaand led to an increase in broad‐sense heritability (h2) and efficiency.ancovawith the second scoring increasedh2from 0.03 for the baseline model to 0.60, whereas the best spatial model increasedh2only up to 0.50. The scoring at Issoudun was not independent from the genotypic effects and reduced phenotypic correlations. We concluded that environmental factors causing spatially differing yield potential can be scored or measured and used as covariates to obtain more precise genotypic estimates.
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