High Expression of Neuropilin-1 Associates with Unfavorable Clinicopathological Features in Hepatocellular Carcinoma
High Expression of Neuropilin-1 Associates with Unfavorable Clinicopathological Features in Hepatocellular Carcinoma
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DOI：
10.1007/s12253-015-0003-z
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发表时间：
2016-04
影响因子：
2.8
通讯作者：
Yafei Zhang;Peng Liu;Yi-Qun Jiang;Xiaofeng Dou;Jianghua Yan;Chao Ma;Qun Fan;Weixing Wang;Fu Su;Hui Tang;X. Su
中科院分区：
文献类型：
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作者：
Yafei Zhang;Peng Liu;Yi-Qun Jiang;Xiaofeng Dou;Jianghua Yan;Chao Ma;Qun Fan;Weixing Wang;Fu Su;Hui Tang;X. Su
神经纤毛蛋白-1（NRP-1）作为血管内皮生长因子（VEGF）的共受体，在多种肿瘤的血管生成和恶性进展中发挥重要作用。然而，NRP-1在肝细胞癌（HCC）中的作用还不清楚。本研究旨在检测Neuropilin-1在肝癌组织中的表达，探讨其表达与肝癌临床病理特征及预后的关系。采用实时定量PCR（qRT-PCR）、Western blot、免疫荧光和免疫组织化学（IHC）分析NRP-1在HCC细胞系和组织中的表达。随后评估NRP-1表达与临床病理特征和预后的关系。qRT-PCR和Western blot检测显示，NRP-1在肝癌组织中的表达明显高于正常肝细胞和正常肝组织（P<0.05和<0.001）。此外，NRP-1的高表达与肝内转移（P= 0.036）、Edmondson分级（P= 0.007）、TNM分期（P= 0.0031）和门静脉浸润（P= 0.004）显著相关。此外，NRP-1高表达的HCC患者的总生存期（OS）和无复发生存期（RFS）较短，而NRP-1低表达的患者的OS和RFS较好（P分别为0.0035和0.0048）。这些数据表明，NRP-1表达可能在HCC的进展中起重要作用，并且NRP-1高表达提示HCC患者的不利临床病理特征和生存。
As a co-receptor for vascular endothelial growth factor (VEGF), Neuropilin-1 (NRP-1) plays an important role in angiogenesis and malignant progression of many human cancers. However, the role of NRP-1 in hepatocellular carcinoma (HCC) is not well understood. The study aimed to detected the expression of Neuropilin-1 in HCC and investigate the association between its expression and the clinicopathological characteristics and prognosis of HCC. Quantitative real-time PCR (qRT-PCR), Western blot, Immunofluorescence and immunohistochemistry (IHC) analyses were performed to characterize the expression of NRP-1 in HCC cell lines and tissues. The association of NRP-1 expression with the clinicopathological characteristics and the prognosis was subsequently assessed. qRT-PCR and Western blot assays revealed that the expression of NRP-1 in HCC was significantly increased relative to that of normal live cells and tissues (P< 0.05,and <0.001, respectively). In addition, high expression of NRP-1 was significantly associated with intrahepatic metastasis (P= 0.036), Edmondson grade (P= 0.007), TNM classification (P= 0.0031), and portal vein invasion (P= 0.004). Furthermore, the HCC patients with high NRP-1 expression had shorter overall survival (OS), and recurrence-free survival (RFS), whereas, patients with low NRP-1 expression had better OS and RFS (P= 0.0035, and 0.0048, respectively). These data indicate that NRP-1 expression may play an important role in the progression of HCC, and that high NRP-1 expression suggests unfavorable clinicopathological characteristics and survival in HCC patients.