INTESTINAL MUCOSAL MAST-CELLS IN NORMAL AND NEMATODE-INFECTED RAT INTESTINES ARE IN INTIMATE CONTACT WITH PEPTIDERGIC NERVES
INTESTINAL MUCOSAL MAST-CELLS IN NORMAL AND NEMATODE-INFECTED RAT INTESTINES ARE IN INTIMATE CONTACT WITH PEPTIDERGIC NERVES
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DOI：
10.1073/pnas.84.9.2975
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发表时间：
1987-05-01
影响因子：
11.1
通讯作者：
BIENENSTOCK, J
中科院分区：
文献类型：
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作者：
STEAD, RH;TOMIOKA, M;BIENENSTOCK, J
过敏性疾病的炎症，以及愈合和修复过程发生的情况，其特征在于存在数量增加的肥大细胞。先前关于神经肽对肥大细胞介质释放的影响的工作表明，只有P物质引起肠粘膜肥大细胞的这种释放[Shanahan，F.，Denburg，J. A.，狐狸. J.，Bienenstock，J.和Befus，A. D. 135，1331-1337]。因此，我们研究了肥大细胞和肠神经在正常大鼠肠道和寄生虫感染的大鼠肠道，其中粘膜肥大细胞增生发生的显微解剖关系。联合免疫组化神经元特异性烯醇化酶和阿辛蓝染色在pH 0.5的石蜡包埋切片正常和日本圆线虫感染的大鼠空肠。67%的肠粘膜肥大细胞接触上皮下神经，另外20个在神经的2 μ m内。 评估肥大细胞占据的固有层比例（12.5%），平均肥大细胞面积（121 ± 0.5%）。28 μ m2）和肠神经密度（每788 ± 28 μ m2一个）。151 μ m2）表明，该关联是仅偶然预期的5倍（P < 0.0001）。在连续切片中，与肥大细胞接触的神经也显示含有P物质和/或降钙素基因相关肽。电子显微镜证实了这一关联：8%的肥大细胞在感染大鼠表现出膜膜接触无髓鞘轴突含有70- 170纳米致密核心囊泡，和另外31%位于小于250 nm的神经。其他肥大细胞似乎通过板足的投射包围神经束。这些数据提供了系统的定量证据，表明在大鼠胃肠道中存在免疫系统和神经系统之间通讯的结构基础。
Inflammatory of allergic conditions, as well as situations where healing and repair processes occur, are characterized by the presence of increased numbers of mast cells. Previous work on the effect of neuropeptides on mast cell mediator release showed that only substance P caused such release from intestinal mucosal mast cells [Shanahan, F., Denburg, J. A., Fox. J., Bienenstock, J. and Befus, A. D. (1985) J. Immunol. 135, 1331-1337]. Accordingly, we investigated the microanatomical relationship between mast cells and enteric nerves in normal rat intestine and parasite-infected rat intestine, in which mucosal mast cell hyperplasia occurs. Combined immunohistochemistry for neuron-specific enolase and staining with alcian blue at pH 0.5 was employed on paraffin-embedded sections of normal and Nippostrongylus brasiliensis-infected rat jejunum. Sixty-seven percent of intestinal mucosal mast cells were touching subepithelial nerves, and an additional 20 were within 2 .mu.m of nerves. Assessment of the proportion of the lamina propria occupied by mast cells (12.5%), the average mast cell area (121 .+-. 28 .mu.m2), and the density of enteric nerves (one per 788 .+-. 151 .mu.m2) suggested that the association was 5 times greater than would be expected by chance alone (P < 0.0001). In consecutive sections, the nerves in contact with mast cells were also shown to contain substance P and/or calcitonin-gene-related peptide. Electron microscopy confirmed this association: 8% of the mast cells in infected rats exhibited membrane-membrane contact with unmyelinated axons containing 70- 170-nm dense-core vesicles, and an additional 31% were situated less than 250 nm from nerves. Other mast cells appeared to embrace nerve bundles through the projection of lamellopodia. These data provide systematic quantitative evidence that a structural foundantion for communication between the immune and nervous systems exists in the rat gastrointestinal tract.