Lesion measurement in non-radioactive DNA by quantitative gel electrophoresis.
Lesion measurement in non-radioactive DNA by quantitative gel electrophoresis.
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通过定量凝胶电泳测量非放射性 DNA 损伤。
DOI：
10.1007/978-1-4613-0637-5_4
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Sutherland,BM
中科院分区：
文献类型：
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作者：
Sutherland,JC;Chen,CZ;Emrick,A;Hacham,H;Monteleone,DC;Ribeiro,E;Trunk,J;Sutherland,BM
DNA 损伤和修复在体内平衡中的重要作用已通过涉及分离 DNA、培养中生长的原核细胞和真核细胞的研究清楚地证明。从这些模型系统中获得的信息虽然非常有价值，但永远无法取代对完整高等生物体数据的需求。我们实验室在过去十年中开发的凝胶电泳方法使得对 UV 诱导的嘧啶二聚体、伽马射线诱导的单链和双链断裂以及许多其他类型的 DNA 损伤进行纳克级定量成为可能。 DNA 不必用放射性核素或特定构象进行标记，因此有助于使用该方法来测量完整有机体（包括人类）DNA 的损伤水平和修复率。
The important role of DNA damage and repair in homeostasis has been clearly demonstrated by studies involving isolated DNA, procaryotic cells and eukaryotic cells growing in culture. The information obtainable from these model systems, while extremely valuable, can never replace the need for data from intact higher organisms. The gel electrophoresis method developed during the past ten years in our laboratories makes possible the quantitation of UV induced pyrimidine dimers, gamma ray induced single- and double-strand breaks and many other types of lesions in nanogram quantities of DNA. The DNA does not have to be labeled with radionuclides or of a particular conformation, thus facilitating the use of the method in measuring damage levels and repair rates in the DNA of intact organisms -- including man.