Quantitative dynamic cellular imaging based on 3D unwrapped optically computed phase microscopy.
Quantitative dynamic cellular imaging based on 3D unwrapped optically computed phase microscopy.
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DOI：
10.1364/ao.463843
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发表时间：
2022-09-20
期刊：
影响因子：
1.9
通讯作者：
Zhang, Yuanwei
中科院分区：
文献类型：
--
作者：
Liu, Xuan;Liu, Yuwei;Wan, Zhaoxiong;Gunasekar, Arun Kumar;Zhang, Yuanwei
我们通过定量相位显微镜研究了动态现象的连续观察。我们使用光学计算相位显微镜进行成像研究，这是我们实验室开发的一种新型成像技术。连续相位成像不可避免地受到相位缠绕伪影的影响，这影响了样品动态的正确量化。为了解决这个问题，我们开发了一种三维展开方法，利用空间和时间上的数据相关性。我们使用模拟数据验证了我们的三维相位展开方法。我们使用实验数据进一步验证了3D相位展开，并展示了定量相位成像，准确表征了样品动态。我们成像的纳米级运动的样品驱动的压电换能器（PZT）。我们使用三维展开相位计算了位移，结果与已知的PZT运动一致。我们还对从培养皿基底上分离的活细胞进行了成像。使用3D展开相位计算的光程长度随着细胞的干质量在分离过程中变得更加集中而增加。
We investigated continuous observation of dynamic phenomena through quantitative phase microscopy. We conducted imaging studies using optically computed phase microscopy, a novel imaging technology developed in our lab. Inevitably, continuous phase imaging is affected by phase wrapping artifact, which affects correct quantification of sample dynamics. To address this issue, we developed a 3D unwrapping method that exploited data correlation in space as well as in time. We validated our 3D phase unwrapping method using simulated data. We further validated 3D phase unwrapping using experimental data and demonstrated quantitative phase imaging that accurately characterized sample dynamics. We imaged the nanoscale motion of the sample actuated by a piezo transducer (PZT). We calculated the displacement using 3D unwrapped phase and the result was consistent with the known motion of the PZT. We also imaged live cells that were detaching from the substrate of the petri dish. The optical path length calculated using 3D unwrapped phase increased as the dry mass of the cell became more concentrated during the detachment process.