Molecular testing of multiple HIV-1 transmissions in a criminal case
Molecular testing of multiple HIV-1 transmissions in a criminal case
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DOI：
10.1097/01.aids.0000187904.02261.1a
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发表时间：
2005-10-14
期刊：
影响因子：
3.8
通讯作者：
Vandamme, AM
中科院分区：
文献类型：
--
作者：
Lemey, P;Van Dooren, S;Vandamme, AM
目的：为验证一起刑事案件中HIV-1从一名嫌疑人传播到六名受者的先验假设，方法：对嫌疑人和受害者的至少两份样本进行部分pol和/或env序列分析。根据流行病学和亚型标准，对适当的当地对照进行采样。系统发育测试进行了不同的重建methods.Results：系统发育分析一致推断的嫌疑人和受害者样本在两个基因组区域的单系聚类。这是高度支持的参数和非参数自举技术。此外，控制最密切相关的嫌疑人，受害者集群有一个类似的地理来源的suspect.Conclusions：考虑到限制的结论，可以从分子调查，我们可以推断，我们的分子数据是一致的情况下，多个艾滋病毒传播的嫌疑人和受害者之间。
Objective: To test the a priori hypothesis of HIV-1 transmission from one suspect to six recipients in a criminal case.Methods: Partial pol and/or env sequences were obtained for at least two samples of the suspect and the victims. Appropriate local controls were sampled based on epidemiological and subtype criteria. Phylogenetic testing was performed using different reconstruction methods.Results: Phylogenetic analyses consistently inferred a monophyletic cluster for the suspect and victim samples in both genome regions. This was highly supported by parametric and non-parametric bootstrapping techniques. Moreover, the controls most closely related to the suspect-victim cluster had a similar geographical origin to the suspect.Conclusions: Taking into account the limitations on the conclusions that can be drawn from molecular investigations we could infer that our molecular data is consistent with a scenario of multiple HIV transmission between suspect and victims.