INVIVO ANALYSIS OF CELLULAR REPLICATION
INVIVO ANALYSIS OF CELLULAR REPLICATION
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DOI：
10.1073/pnas.74.5.2041
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发表时间：
1977-01-01
影响因子：
11.1
通讯作者：
TICE, RR
中科院分区：
文献类型：
--
作者：
SCHNEIDER, EL;STERNBERG, H;TICE, RR
中期细胞在BrdUrd [溴脱氧尿苷]存在下所经历的先前细胞复制的数量可以通过用Hoechst染料33258染色的中期染色体的差异荧光图案来确定。为了检查这种技术是否可以应用于分析体内细胞周期动力学，Wistar大鼠输注BrdUrd 7.5-33小时，浓度的核苷酸类似物，不抑制细胞复制。检查1、2和3个或更多个复制周期细胞的频率作为BrdUrd输注时间的函数，表明大鼠骨髓细胞的细胞复制时间相对均匀。用计算机模拟模型对这些数据进行分析，得出平均细胞周期持续时间为9.2 h，这与放射性同位素研究获得的最快时间一致。这些结果支持的潜力，非放射性同位素分析的细胞复制在体内。
The number of previous cell replications that a metaphase cell has undergone in the presence of BrdUrd [bromodeoxyuridine] can be determined by the differential fluorescent patterns of metaphase chromosomes stained with Hoechst dye 33258. To examine if this technique could be applied to analyzing cell cycle kinetics in vivo, Wistar rats were infused with BrdUrd for 7.5-33 h at concentrations of the nucleotide analog that did not inhibit cellular replication. Examination of the frequency of 1, 2, and 3 or more replication cycle cells as a function of BrdUrd infusion time indicates that cell replication times for rat bone marrow cells are relatively homogeneous. Analysis of this data with a computer simulation model produced a mean cell cycle duration of 9.2 h, which is compatible with the fastest times obtained with radioisotope studies. These results support the potential of nonradioisotope analysis of cell replication in vivo.