Dendrite-specific remodeling of Drosophila sensory neurons requires matrix metalloproteases, ubiquitin-proteasome, and ecdysone signaling
Dendrite-specific remodeling of Drosophila sensory neurons requires matrix metalloproteases, ubiquitin-proteasome, and ecdysone signaling
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DOI：
10.1073/pnas.0507393102
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发表时间：
2005-10-18
影响因子：
11.1
通讯作者：
Jan, YN
中科院分区：
文献类型：
--
作者：
Kuo, CT;Jan, LY;Jan, YN
在神经元成熟过程中，树突从未成熟的神经突发育成成熟的乔木。由于环境的变化，某些成熟神经元的树突可以进行大规模的形态重塑。在这里，我们展示了一组果蝇周围感觉神经元，即IV类树突树突（C4da）神经元，它们完全降解并再生其精致的树突。C4da神经元的幼体树突首先从体细胞中分离出来，随后在变态过程中降解。这一过程受到细胞内和细胞外机制的控制：蜕皮激素途径和泛素蛋白酶体系统（UPS）是启动树突断裂的细胞内在信号，细胞外基质金属蛋白酶需要降解被切断的树突。令人惊讶的是，尽管激活了蜕皮激素和UPS通路，C4da神经元在并发树突降解过程中仍保持轴突投射。这些结果表明，在对环境变化的反应中，某些神经元具有完全失去树突但保留轴突并随后重新生长树突乔木的细胞内在能力。
During neuronal maturation, dendrites develop from immature neurites into mature arbors. In response to changes in the environment, dendrites from certain mature neurons can undergo large-scale morphologic remodeling. Here, we show a group of Drosophila peripheral sensory neurons, the class IV dendritic arborization (C4da) neurons, that completely degrade and regrow their elaborate dendrites. Larval dendrites of C4da neurons are first severed from the soma-and subsequently degraded during metamorphosis. This process is controlled by both intracellular and extracellular mechanisms: The ecdysone pathway and ubiquitin-proteasome system (UPS) are cell-intrinsic signals that initiate dendrite breakage, and extracellular matrix metalloproteases are required to degrade the severed dendrites. Surprisingly, C4da neurons retain their axonal projections during concurrent dendrite degradation, despite activated ecdysone and UPS pathways. These results demonstrate that, in response to environmental changes, certain neurons have cell-intrinsic abilities to completely lose their dendrites but keep their axons and subsequently regrow their dendritic arbors.