Exploring the relationship between active bacterioplankton and phytoplankton in the Southern Ocean
Exploring the relationship between active bacterioplankton and phytoplankton in the Southern Ocean
复制标题
探索南大洋活跃浮游细菌和浮游植物之间的关系
DOI：
10.3354/ame01216
复制
发表时间：
2008
影响因子：
1.4
通讯作者：
C. Duarte
中科院分区：
文献类型：
--
作者：
E. Ortega‐Retuerta;I. Reche;E. Pulido;S. Agustí;C. Duarte
浮游细菌是由不同生理状态的细胞组成的异质群落。在南极半岛周围的沃茨中，对浮游细菌的活性部分作为影响南大洋细菌和浮游植物之间关系强度的潜在因素的考虑进行了评估。我们通过吸收3 H-亮氨酸（细菌生产（BP））估计了活性浮游细菌，并根据其相对核酸含量（高（HNA）与低（LNA）），使用活体染色估计其在总浮游细菌群落中的比例，并根据其膜渗透性通过核酸双染色（NADS）估计。并对该海域浮游细菌总量、活性和叶绿素a进行了对比分析。NADS染色表明，所有细菌中有61%是活的，这比以前报道的南大洋细菌总数的比例更高。HNA细菌占所有细菌的45%，表明16%的细菌可能是活的，但具有LNA。BP与LNA细胞丰度的相关性比NADS活菌或HNA细菌更强。当单独考虑HNA或NADS活细胞的丰度时，叶绿素a和细菌丰度（BA）之间的关系并没有增加，表明染色剂的活力/活性并没有增强BA和南大洋浮游植物生物量之间的联系。与此相反，叶绿素a和BP之间的关系比文献报道的更强，这表明，在这一地区，BP是密切依赖于浮游植物。
Bacterioplankton are a heterogenous community composed of cells with different phys- iological states. The consideration of the active fraction of bacterioplankton as a potential factor affecting the strength of the relationship between bacteria and phytoplankton in the Southern Ocean was evaluated in waters around the Antarctic Peninsula. We estimated active bacterioplankton from uptake of 3 H-Leucine (bacterial production (BP)) and using vital stains to estimate their proportion within the total bacterioplankton community, based on their relative nucleic acid content (high (HNA) vs. low (LNA)), and by nucleic acid double staining (NADS), based on their membrane perme- ability. Then we performed a comparative analysis between total and active bacterioplankton and chlorophyll a (chl a) in this area. Staining with NADS suggested that 61% of all bacteria were viable, a higher proportion of the total bacterial community than previously reported for the Southern Ocean. HNA bacteria comprised 45% of all bacteria, indicating that 16% of bacteria may be viable but with LNA. BP was more strongly related to abundance of LNA cells than NADS-viable or HNA bacteria. The relationship between chl a and bacterial abundance (BA) did not increase when consid- ering the abundance of HNA or NADS-viable cells alone, showing that viability/activity of stains did not enhance the linkage between BA and phytoplankton biomass in the Southern Ocean. In contrast, the relationship between chl a and BP was stronger than those reported in the literature, suggesting that, in this region, BP is closely dependent on phytoplankton.