Syndecan-4 is a primary-response gene induced by basic fibroblast growth factor and arterial injury in vascular smooth muscle cells
Syndecan-4 is a primary-response gene induced by basic fibroblast growth factor and arterial injury in vascular smooth muscle cells
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DOI：
10.1161/01.atv.17.1.172
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发表时间：
1997-01-01
影响因子：
8.7
通讯作者：
Carey, DJ
中科院分区：
文献类型：
--
作者：
CizmeciSmith, G;Langan, E;Carey, DJ
多配体聚糖是一个跨膜蛋白聚糖家族，参与细胞-细胞外基质粘附和生长因子结合。我们以前报道，syndecan-1表达培养的大鼠血管平滑肌细胞（VSMCs）诱导的血清或血小板衍生生长因子（PDGF）。我们现在报告，syndecan-4 mRNA在培养的VSMCs中快速诱导，以响应碱性成纤维细胞生长因子（bFGF）或血清刺激。在放线菌酮的存在下，syndecan-4 mRNA的诱导增强。这些特征确定syndecan-4作为VSMCs中的初级反应基因产物。与此相反，syndecan-1的mRNA表达在血清中的放线菌酮的存在下被完全阻断。我们还研究了多配体蛋白聚糖mRNA在血管平滑肌细胞中的表达，以响应体内球囊导管损伤。逆转录-聚合酶链反应技术的开发，使我们能够在一个反应管中扩增所有四个syndecan mRNA，并确定其表达的相对变化。在未损伤的大鼠颈动脉中检测到所有四种多配体蛋白聚糖mRNA。在内皮剥脱的动脉中，中层（推测为VSMC）占血管壁中syndecan mRNA的70%至90%。球囊损伤后syndecan-2和syndecan-3的mRNA水平没有显著改变。与此相反，syndecan-4 mRNA在损伤后早期增加，但随后下降到对照组水平7天。Syndecan-1 mRNA水平表现出缓慢但延长的增加，在损伤后7天达到最大值。用抗syndecan-4抗体的免疫染色表明，在受损的颈动脉中syndecan-4蛋白聚糖表达迅速增加。
Syndecans are a family of transmembrane proteoglycans that have been implicated in cell-extracellular matrix adhesion and growth factor binding. We reported previously that syndecan-1 expression by cultured rat vascular smooth muscle cells (VSMCs) is induced by serum- or platelet-derived growth factor (PDGF). We now report that syndecan-4 mRNA is rapidly induced in cultured VSMCs in response to basic fibroblast growth factor (bFGF) or serum stimulation. In the presence of cycloheximide, induction of syndecan-4 mRNA was enhanced. These characteristics identified syndecan-4 as a primary-response gene product in VSMCs. In contrast, syndecan-1 mRNA expression in response to serum was completely blocked in the presence of cycloheximide. We also examined the expression of syndecan mRNAs in VSMCs in response to balloon catheter injury in vivo. A reverse transcriptase-polymerase chain reaction technique was developed that enabled us to amplify all four syndecan mRNAs in a single reaction tube and determine relative changes in their expression. All four syndecan mRNAs were detected in uninjured rat carotid arteries. In endothelium-denuded arteries, the medial layer (presumably VSMCs) accounted for 70% to 90% of the syndecan mRNAs in the vessel wall. The levels of syndecan-2 and syndecan-3 mRNAs were not altered significantly after balloon injury. In contrast, syndecan-4 mRNA was increased at early times after injury but then decreased to control level by 7 days. Syndecan-1 mRNA levels showed a slower but prolonged increase that reached a maximum at 7 days after injury. Immunostaining with anti-syndecan-4 antibodies demonstrated a rapid increase in syndecan-4 proteoglycan expression in the injured carotid artery.